摘要
【目的】研究大豆异黄酮(Soybean isoflavone,SI)的抑菌机制。【方法】本试验利用呼吸代谢抑制实验和4',6-二脒基-2-苯基吲哚(4',6-diamidino-2-phenylindole,DAPI)荧光染色对大豆异黄酮的抑菌机制进行研究。【结果】呼吸代谢抑制实验结果表明,当SI的终浓度为0.8mg·ml-1时,可显著抑制金黄色葡萄球菌苹果酸脱氢酶的活性,SI组苹果酸脱氢酶的比活性与对照组相比降低了74.97%。DAPI染色结果显示,DAPI可以和金黄色葡萄球菌内的核酸结合,经过SI作用28h后,菌体内的核酸含量呈明显下降趋势,其中DNA的荧光强度减少了33.53%,RNA减少了39.82%。【结论】SI具有有较强的抑菌活性,其抑菌机制是通过抑制细菌的呼吸代谢和核酸的合成等方面实现的。
[ Objective ] To investigate the anti-bacterial mechanism of soybean isoflavone （SI） and provide a theoretical basis for the development of high-performance and low toxicity of antibacterial drugs. [Method] Respiratory metabolism test and 4＇6-diamidino-2-phenylindole （DAPI） staining assay were used to investigate the antibacterial mechanism of SI. [Result] The results indicated that the malic dehydrogenase （MDH） activity of Staphylococcus aureus was significantly inhibited by 0.8 mg·ml^-1 SI and the malic dehydrogenase （MDH） activity of Staphylococcus aureus was remarkably reduced by 74.97% compared to the control group. The fluorescence microscopy and fluorescence spectrophotometer studies manifested that 4＇6-diamidino-2-phenylindole （DAPI） could integrate with Staphylococcus aureus, and the quantity of DNA and RNA were significantly reduced by 33.53% and 39.82% after incubation with SI for 28 hours. [Conclusion] The results showed that SI could inhibit the growth of curtain bacteria significantly, the mechanisms of the inhibition activity may include inhibiting both respiratory metabolism and synthesis of nucleic acid.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第7期2586-2591,共6页
Scientia Agricultura Sinica
基金
辽宁省自然科学基金(20072153)
作者简介
王海涛（1982-），男，河北昌黎人，硕士研究生，研究方向为微生物生化。E-mail：whtbob@163．com。
通信作者谢明杰（1963一），女，辽宁大连人，教授，博士，研究方向为微生物催化采油、微生物肥料、微生物酶、生物活性物质的提取和分离纯化及其作用机制等。Tel：0411-84259341；E-mail：xmj1222@sina．com
