摘要
建立并改进了植物血红素加氧酶（heme oxygenase，HO）活性的微量比色测定法。测定体系的最佳反应条件为：最适pH7．2，最适温度37℃，加入5～20μL酶液量（总蛋白量控制在5～10μg），酶促反应时间为60min。并通过比较正常生长和盐胁迫条件下多种植物的HO活性变化，初步证明HO具有盐胁迫诱导特性。
A micro-volume spectrometry for determination of heme oxygenase （HO） activity in plants was established and further modified. The optimum conditions for determination were as follows： the optimum pH and temperature were 7. 2 and 37 ℃, the applied volume of extract was about 5 -20 μL （equivalent to 5 - 10 μg protein） , and 60 min was chosen as reaction time. Finally, in comparison with the changes of HO activities under normal growth condition or salt stress in several plant species as reaction time, we also preliminarily discovered that HO exhibits the salt-inducible character.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2009年第3期146-150,共5页
Journal of Nanjing Agricultural University
基金
国家自然科学基金项目(30471049
30671248)
国家基础科学人才培养基金项目(J0730647)
江苏省自然科学基金项目(BK2007157)
南京农业大学SRT项目(0706A01)
关键词
酶活性测定
血红素加氧酶
盐胁迫诱导特性
determining enzyme activity
home oxygenase
salt-inducible character
作者简介
作者简介：陈舟舟，本科生。
通讯作者：沈文飚，教授，从事植物信号转导方面的研究，E-mail：wbshenh@njau．edu．cn Tel：025-84398602。
