摘要
目的研究新近报道与丙氨酸转氨酶异常相关的ＴＴＶ在已知和未知病毒性肝炎中的临床意义。方法设计ＴＴＶ部分基因的特异性引物，用聚合酶链反应（ＰＣＲ）方法检测了１０４例病毒性肝炎的ＴＴＶＤＮＡ，并对１例ＴＴＶ阳性标本克隆测序。结果ＴＴＶＤＮＡ序列与日本ＴＴＶ部分基因序列相对应位置的核苷酸同源性为９８．４％。在１０４例肝炎患者中ＴＴＶＤＮＡ阳性检出率为２４．０％（２５／１０４），其中在非甲～戊和庚型肝炎患者中为４８．０％（１２／４２），在甲型肝炎中为１９．０％（４／２１），乙型肝炎为２５．０％（８／３２），丙型肝炎为１１．１％（１／９），９例ＨＧＶＲＮＡ阳性者中未检出ＴＴＶＤＮＡ。值得注意的是重型肝炎ＴＴＶ检出率极高，其中急性重型肝炎６例中阳性为４例（６６．７％），慢性重型肝炎６例中阳性为３例（５０．０％）。结论ＴＴＶ在我国肝炎病人中存在。在重型肝炎中有较高的发生率，可能是未知病毒致急性和慢性及重型肝炎的病因之一。
104 serum samples from patients with liver diseases were detected for TTV DNA by nested-PCR method.The results showed that 25 of 104(24%) were positive for TTV DNA.Among 104 samples,48%(12/42) were positive for nonA-E and G 19%(4/12)were positive for hepatitis A 25%(8/32)were positive hepatitis B 11.1%(1/9)was positive hepatitis C 4 of 6(66.7%) fulminant hepatitis cases were detected positive for TTV DNA.The partial gene of two TTV isolates from the patients were cloned and sequenced. The sequence analysis shows that there is 98.4% nucleotide identity between AB008394(from Japan)and one isolate from the patient (TTVEC023).The results suggest that TTV is associated with acute, chronic and fulminant hepatitis.TTV infection would be the important cause of non A-E and G hepatitis.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1998年第2期111-114,共4页
Chinese Journal of Experimental and Clinical Virology
关键词
TTV
聚合酶链反应
肝炎
核苷酸序列测定
Transfusion transmitted virus Polymerase chain reaction Nucleotide sequence Hepatitis
