摘要
目的研究新生隐球菌共孵育后血管内皮细胞与正常细胞的差异蛋白质谱,推测蛋白质表达改变在孵育过程中的作用。方法利用二维凝胶电泳获得新生隐球菌孵育后血管内皮细胞与正常细胞的差异表达蛋白点,对部分差异蛋白点进行质谱鉴定分析,并以实时荧光定量PCR对其mRNA表达进行定量比较。结果Peroxiredoxin Ⅰ及Calpactin Ⅰ light chain等13个蛋白的表达水平发生明显改变,Peroxiredoxin Ⅰ及Calpactin Ⅰlight chain的mRNA表达明显改变,其mRNA含量的改变趋势与相应的蛋白质的变化趋势相同。结论Peroxiredoxin Ⅰ及Calpactin Ⅰ light chain等蛋白表达量的改变可能与新生隐球菌侵袭血管内皮细胞屏障有关。
Objective To study the differences of protein profile between HUVEC incubated with Cryptococcus neoformans and normal HUVEC,and to speculate its role during incubation. Methods The expression proteins between HUVEC incubated with Cryptococcus neoformans and normal HUVEC were obtained by 2-D electrophoresis and the differences were identified by MALDI- TOF MS technology, mRNA was quantitated by Real-time q-PCR. Results The expression level of 13 kinds of protein changed evi- dently,especially Peroxiredoxin I and Calpactin I light chain,with corresponding changes of mRNA quantity. Conclusion Expression changes of Peroxiredoxin I and Calpactin I light chain may have relationship with the invasion of Cryptococcus neoformans into the barrier of HUVEC.
出处
《中国真菌学杂志》
2009年第3期129-133,共5页
Chinese Journal of Mycology
基金
国家自然科学基金(30471566
30570097)
关键词
二维凝胶电泳
新生隐球菌
血管内皮细胞
蛋白质组学
实时荧光定量PCR
two-dimensional gel eleetrophoresis
Cryptococcus neoforrrmns
HUVEC
proteomics
real-time fluorescent quantitative PCR
作者简介
汪晓军，男（汉族），博士，主治医师．E-mail：wxjyh@yahoo．com．cn
通讯作者：温海，E-mail：wenhai98@sohu．com
