摘要
[Objective] This study was to investgate the effects of lysine on the growth of sheep and its mechanism. [Method] Fifteen sheep about one-year old as experimental material were divided into three groups （group A, group B end group C）, into whose basal feed 0, 4 and 10 g h/sine were respectively added. After 28 d of feeding, the experimental sheep were all slaughtered for sampling; then total RNAs were extrac- ted from the samples and used to clone GHR and GAPDH genes via retrotrenscription for analyzing the expression abundance of GHR mRNA in Iongissimus dorsi muscle from different treatments. [Result] The expression of GHR mRNA in treatment B was significantly higher than that in treatment A（ P 〈0.01 ）, and significantly higher then that in treatment C （ P 〈0.05） ; while that in treatment C and treatment A was insignificantly different（ P 〉 0.05）. [ Conclusion] Addition of lysine into basal feed of sheep could dose-independently improve the expression of GHR gene in Iongissimus dorsi muscle.
1材料与方法1．1试验材料1．1．1试验动物及分组。选用年龄约1岁，体重约40kg，身体健康的母羊15只。设3个处理组：A对照组（基础日粮）、B赖氨酸低剂量组（基础日粮＋4g赖氨酸盐）和C赖氨酸高剂量组（基础日粮＋10g赖氨酸盐），每组5个重复。试验预试期7d，正试期28d，共计35d。
基金
中央级公益性科研院所基本科研业务费专项资金项目(BRF070104)~~
作者简介
李建升（1981-），男，山东烟台人，硕士研究生，研究方向：动物营养与饲料科学。通讯作者。
