摘要
为了解重庆地区动物源大肠杆菌产超广谱β-内酰胺酶(ESBLs)及头孢菌素酶(AmpC)基因型的分布,利用初筛及表型确认方法,从临床分离的192株仔猪白痢大肠杆菌、65株奶牛乳腺炎大肠杆菌、69株鸡大肠杆菌中筛选出19株产ESBLs、6株产AmpC酶大肠杆菌,并应用PCR扩增及PCR产物测序方法进行ESBLs及AmpC基因型分析。结果显示,重庆地区动物源大肠杆菌携带TEM型、CTX-M型、DHA-1型和CMY-2型基因的阳性率分别为2.15%、5.21%、0.61%和0.61%。其中,有5株同时携带2种基因。而DHA-1型基因为国内兽医临床首次检出,获得GenBank登录号为FJ386455。结果提示,重庆地区以TEM型和CTX-M型β-内酰胺酶为主,且产ESBLs与AmpC酶大肠杆菌为多重耐药菌株,需引起兽医临床的高度重视。
To investigate the genotype distribution of ESBLs and AmpC β-lactamases among Escherichia coli isolated from farm animals in Chongqing City, 192 strains of E. coli isolated from piglets with diarrhea, 65 strains of E. coli isolated from cattles with mastitis and 69 strains of E. coli isolated from chickens were detected. 19 strains of E. coli-producing ESBLs and 6 strains of E. coli-producing AmpC β-lactamases were screened by preliminary screening and phenotypic confirming, and genotype distribution of ESBLs and AmpC β-lctamases were analyzed by PCR and sequencing. In Chongqing area, the genotype positive rate of TEM, CTX-M, DHA-1 and CMY-2 was 2.15%, 5.21%, 0.61% and 0.61% among E. coli isolated from farm animals. Of which, 5 strains of E. coli were found to carry two kinds of genotype. But, DHA-1 genotype was first detected from veterinary clinic in China, its GenBank accession number was FJ386455. The results indicated that TEM and CTX-M are major genotype in Chongqing, and E. coli-produeing β-lactamases had multi-resistance, it deserve more attention in veterinary clinic.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第6期898-903,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
重庆市自然科学基金项目(CSTC
2007BB0002)
作者简介
作者简介：张珍珍（1983-），女，汉族，河北邯郸人，硕士生，E—mail：ywjzzz888@163．com，主要从事兽医药理与毒理学研究通讯作者：吴俊伟，E-mail：wjw999@163．com
