摘要
在特异性和敏感性试验的基础上,组装副猪嗜血杆菌PCR诊断试剂盒,进行批间和批内试剂盒的检测、试剂盒在不同温度条件下的保存期试验等。结果表明,试剂盒特异性好、敏感性高,批内和批间的试剂盒无差异,试剂盒在-20℃可保存30个月,4℃可保存12个月。适用于不同地区、不同条件的实验室快速检测副猪嗜血杆菌。
On the basis of the specificity and sensitivity of PCR for haemophilus parasuis, the PCR diagnosis kit was developped. The specificity, sensitivity, repeatability, the same and different batch kit were checked. Also the different temperature storage life of the kits were evaluated. The result showed that the kits had very good specificity, sensitivity and repeatablity. There were no difference between the same or different batches of PCR kits. The storage life of the kits is 30 months at -20℃and 12 months at 4℃. The kits can be used in different laboratory to check quickly haemophilus parasuis.
出处
《动物医学进展》
CSCD
北大核心
2009年第6期10-14,共5页
Progress In Veterinary Medicine
基金
北京市科委科技项目
作者简介
龚玉梅（1961-）,女，湖南华容人，高级兽医师，主要从事动物疫病诊断与预防工作。
通讯作者
