摘要
目的探讨醒脑静改善脑出血后脑水肿的作用机制，及其对血肿周围基质金属蛋白酶-9和补体C3的影响。方法72只健康雄性SD大鼠，随机分为假手术组、出血组和醒脑静组。自体血注射法建立脑出血模型，Garcia法评价神经功能；干湿重法检测脑组织含水量；根据伊文蓝浓度评价血-脑屏障通透性；免疫组织化学检测血肿周围脑组织中基质金属蛋白酶-9和补体C3表达水平。结果经醒脑静治疗后，大鼠神经功能评分逐渐增加（均P〈0．05），至手术后72h达峰值水平（P〈0．05），且手术后48和72h神经功能评分均高于出血组（P〈0．05）。手术后72h，出血组大鼠脑组织含水量升高，血-脑屏障通透性增加，基质金属蛋白酶-9和补体C3表达阳性细胞数目增多（均P〈0．05）；经醒脑静治疗后，脑组织含水量降低，血-脑屏障通透性下降，基质金属蛋白酶-9和补体C3表达阳性细胞数目减少，与出血组比较差异有统计学意义（均P〈0．05）。结论醒脑静通过下调血肿周围脑组织中基质金属蛋白酶-9和补体C3表达水平而达到保护血-脑屏障、减轻血管源性脑水肿的作用。
Objective To explore the mechanism of xingnaojing on the improvement of cerebral edema after intracerebral hemorrhage （ICH） and the effect on the expressions of matrix metaltoproteinase-9 （MMP-9） and complement 3 （C3） around hematoma. Methods A total of 72 healthy male Sprague-Dawley （SD） rats were randomly divided into 3 groups, sham-operation group （n = 24）, ICH group （n = 24） and ICH with xingnaojing treatment group （XNJ group, n = 24）. The ICH model was established by autoblood injection. Neurofunction was examined immediately at waking after anesthesia and at 24, 48 and 72 h after operation by Garcia method. The water content was assessed by dry and wet method. Evans blue （EB） density was examined to determine the permeability of blood-brain barrier （BBB）. The expressions of MMP-9 and C3 around hematoma were measured with immunohistochemistry. Results After treating by xingnaojing, the neurofunction scores were gradually increased （P〈 0.05, for all）, and achieved to the peak at 72 h after operation （P〈 0.05）. At 48 and 72 h after operation, neurofunction scores in XNJ group were significantly higher than those in ICH group （P〈0.05, for all）. At 72 h after operation, the water content in brain tissue, the permeability of BBB, and the number of cells with positive expression of MMP-9 and C3 increased （P〈 0.05, for all）, and after xingnaojing treatment, the water content, the permeability of BBB, and the number of cells with positive expressions of MMP-9 and C3 decreased. There were significant differences between XNJ group and ICH group （P〈0.05, for all）. Conclusion It is suggested that destruction of blood-brain barrier can induce cerebral edema after intracerebral hemorrhage. Xingnaojing may protect blood-brain barrier and alleviate cerebral edema by decreasing the expressions of MMP-9 and C3.
出处
《中国现代神经疾病杂志》
CAS
2009年第3期284-289,共6页
Chinese Journal of Contemporary Neurology and Neurosurgery
关键词
脑出血
安宫牛黄丸
明胶酶类
补体3
疾病模型
动物
Cerebral hemorrhage
Angong niuhuang pill
Gelatinases
Complement 3
Disease models, animal
作者简介
通讯作者：周广喜（Email：zhougx8888@126．com）
