摘要
目的探讨适合人成纤维样滑膜细胞(FLS)低温冷藏保存的最佳冻存时间。方法将FLS经-80℃冰箱冷冻保存。在冷冻后2、3、4、5wk分别复苏,培养。观察FLS的生长情况和细胞存活率及24h贴壁率。结果冻存5wk的FLS细胞存活率(66±1.3)%,较冻存2wk(90±2.0)%有明显下降;冻存5wk的FLS24h贴壁率(61.3±2)%,与冻存2wk(85.9±3)%比较有明显下降;未冻存和冻存2wk复苏后FLS的增殖能力无明显差异。结论FLS可以进行低温冷冻,冻存时间4wk为宜。
Aim To determine appropriate length of cryopreservation time for fibroblast-like synoviocytes. Methods FLS were cryopreserved in - 80℃ refrigerator, resuscitated and cultured after being frozed for 2,3,4 and 5 weeks. The cell viability and 24 hours adherence rate were evaluated. Results There was significant difference in the rate of cell livability of FLS cryopreserved for 5 weeks compared with that for 2 weeks. Significant difference was also observed in the 24 hours adherence rate of FLS cryopreserved for 5 weeks compared with that for 2 weeks. There was no difference in the proliferation of FLS routinely maintained compared with those cryopreserved for 2 weeks. Conclusions FLS can be cryopreserved and the time limit of cryopreservation should be 4 weeks.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2009年第6期828-830,共3页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30572356)
安徽省科技攻关计划项目(No06013133B)
高等学校博士学科点专项科研基金(No20060366003)
高校首批科技创新团队基金(No2005TD003)
安徽省研究实验基地优秀中青年科研带头人基金(No200622805)
安徽医科大学校科研基金(No2008kj13)
关键词
人成纤维样滑膜细胞
冻存时间
活性
fibroblast-like synoviocytes of human
length of cryopreservation time
viability
作者简介
戴杏（1979-），女，硕士生，E-mail：dx7906@yahoo．cn.
魏伟（1960-），男，教授，博士生导师，通讯作者，E—mail．wwei@ahmu．edu．cn
