摘要
采用31对SRAP引物对80份木薯种质,包括本所从南美、泰国和非洲引进的76份木薯种质,以及华南系列的4个木薯品种,进行遗传多样性分析,获得207个扩增位点,其中多态性位点201个,平均多态性水平为97.1%,每对引物检测等位基因3～11个,平均为6.7个,扩增产物的片段大小范围在250～1 750 bp之间。根据品系间的遗传相似系数,利用UPGMA法进行聚类分析,以遗传相似系数0.635为阈值,将80个木薯品系分为4类群,分别包含38,6,33和3个品系。群体的基因杂合度为0.3 201,遗传多样性指数为0.4 766;群体内的平均多样性指数为0.2762,遗传分化系数为0.2181,基因流系数为1.7 921。实验结果表明,引进的国外资源丰富了我国木薯种质库,拓宽了中国木薯遗传育种的物质基础。
An analyses of the genetic diversity in 80 cassava accessions, among which 76 were introduced from South America, Thailand and African, and 4 varieties belong to South China series, by using 31 pairs of SRAP markers. 207 loci were obtained totally, and 201 were polymorphic loci, the average polymorphism level was 97.1%, 3 to 11 alleles were detected by each pair of primer, with an average of 6.7, the fragment size of PCR products ranging from 250bp to 1750hp. According to the genetic similarity coeffi- cient among different strains and the UPGMA method, with the similarity coefficient criteria of 0.635 these 80 accessions were clustered into 4 groups, which consist of 38, 6, 33 and 3 strains respectively. The value of some parameters are as following： the population heretozygosity （Hi） was 0.3201, the genetic diversity index was 0.4766, the mean value of the population genetic diversity was 0.2762, the coefficient of genetic differentiation was 0. 2181 and the coefficient of gene flow was 1. 7921. These results indicated that the introduction of foreign resources enriched cassava germplasm bank in China, and expanded our country＇s cassava breeding basis.
基金
中国热带农业科学院热带作物品种资源研究所研究生科研基金(YJS-2008-S011)
作者简介
周建国（1978-），男，海南儋州人，作物遗传育种专业在读研究生，从事分子育种研究
通讯作者：李开绵（1964-），男，研究员，中国木薯首席专家
