摘要
利用常规肌肉营养测试方法测定分析了10尾野生点篮子鱼(Siganus guttatus)的一般生化成分与氨基酸含量。结果表明,点篮子鱼肌肉(鲜样)中水分、粗灰分、粗蛋白和粗脂肪的质量分数分别为74.27%、1.60%、21.20%和2.79%。点篮子鱼肌肉中检测出18种氨基酸(包括牛磺酸,胱氨酸未检测),总量为74.57%(质量分数,干样),必需氨基酸总量为31.72%,占氨基酸总量的42.54%,点篮子鱼必需氨基酸的构成比例符合联合国粮农组织/世界卫生组织(FAO/WHO)的标准。点篮子鱼的限制性氨基酸为色氨酸和(蛋氨酸+胱氨酸),其必需氨基酸指数(IEAA)为69.77。点篮子鱼肌肉中4种鲜味氨基酸(DAA)总量为25.41%(质量分数,干样),占总氨基酸含量的34.01%。对点篮子鱼肌肉10种必需氨基酸的A/E值进行了计算与分析,从A/E值的大小次序来看,点篮子鱼与银大麻哈鱼基本类似,比值最高的前三位必需氨基酸依次为赖氨酸、亮氨酸和精氨酸,最小的为色氨酸。A/E值为点篮子鱼必需氨基酸需求研究和配合饲料的开发提供了基础素材。
Biochemical components and amino acids composition in the muscle of Siganus guttatus were tested and analyzed with routine methods. Samples of 10 wild individuals, with （20.04 ± 0.53） cm body length and （305.86 ± 34.00） g body weight, were collected from the South China Sea, near Sanya City of Hainan Province in September of 2007. The results showed that contents of moisture, crude ash, crude protein and crude fat of fresh muscles were 74.27 %, 1.60 % ,21.20 % and 2.79 %, respectively. The composition of amino acids of muscle was 18 common amino acids, including Tau, and 8 essential amino acids for human needs （no analysis was made for Cys）. In dry sample the total content of amino acids was 74.57%; the content of essential amino acids was 31.72% ; the percentage of essential amino acids in total amino acids was 42.54%. It was apparent that the content of the different amino acids was stable and the constitutional rate of the essential amino acids accorded with the FAO/WHO Standard, fundamentally. According to nutrition evaluation in amino acids score （SAA） and chemical score （Sc ）, the first limited amino acid was Trp, and the second limited amino acids was Phe ＋ Tyr; the essential amino acids index （ IEAA ） was 69.77 ; in dry sample the content of four kinds delicious amino acids was 25.41%, and the percentage of delicious amino acids in total amino acids were 34.01%. We calculated the AlE ratios of l0 essential amino acids for S. guttatus. Based on the descent order of A/E ratios, the first three essential amino acids were Glu, Leu and Arg. The value of A/E ratio for Trp was the smallest in 10 essential amino acids. This descent order was also found in chum salmon. A/E ratios of S. guttatus provided basic materials for research of essential amino acids requirement and artificial food development.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2009年第3期308-313,共6页
Journal of Shanghai Ocean University
基金
国家“十一五”科技支撑计划项目(2006BAD03B08-08)
国家“八六三”高技术研究发展计划项目(2008AA10Z227)
上海市教委E-研究院建设项目(E03009)
关键词
点篮子鱼
肌肉
生化成分
氨基酸
营养评价
A/E值
Siganus guttatus
muscle
biochemical components
amino acid
nutritive evaluation
A/E ratio
作者简介
赵峰（1978-），男，山东德州人，助理研究员，主要从事鱼类生态生理及繁育生物学研究。E-mail：jorfingshfu@hotmail．com.
通讯作者：章龙珍，E-mail：longzhen2885@hotmail．com.
