摘要
目的:研究千里光浸提液的抗氧化活性和黄酮提取量。方法:通过邻二氮菲-Fe2+/H2O2体系分光光度法和光照核黄素分光光度法测定浸提液对羟自由基(OH)和超氧自由基(.O2)的清除率来考查千里光浸提液的抗氧化作用;通过碱性条件下NaNO2-AlNO3显色、芦丁对照分光光度法测定浸提液的总黄酮含量。结果:千里光提取液具有较强的清除超氧自由基和清除羟自由基的作用,其中水提液清除超氧自由基的抗氧化活性较好,醇提液清除羟自由基的抗氧化活性较好,总黄酮提取量水提液比醇提液高,可达31.15mg/g。结论:千里光提取液具有较强的抗氧化活性和较高的黄酮含量。
Purpose : To study the antioxidant activity of senecio extract and the volume of flavonoid extraction. Methods : Throughthephenanthroline-Fe2 +/ H2O2systemspectrophotometry and light riboflavin spectrophotometry,the rate of the extraction for the hydroxylradical (OH) and superoxide radical(.O2)clearance was determined to examine the antioxidation of senecio extract . Through the NaNO2-Al NO3color andrutincontrol spectrophotometryinalkaline conditions ,the content of total flavonoids extract was determined.Results :Theexperi mental results showed that the senecio extract had a strong effect onthe removal of superoxide radical and hydroxyl radical ,in whichwater extract is better for the antioxidant activity of the removal of superoxide radical and alcohol extract is better for the removal ofhydroxyl radical . The volume of the total flavonoids extract was that the water extract was higher thanthe alcohol extract ,upto 31 .15mg/g.Conclusion: The senecio extract has a strong antioxidant activity and a higher content of flavonoids .
出处
《云南中医中药杂志》
2009年第5期51-52,共2页
Yunnan Journal of Traditional Chinese Medicine and Materia Medica
关键词
千里光
提取液
总黄酮含量
抗氧化活性
senecio
extract
clearance rate
content of total flavonoids
