摘要
建立了LC-MS/MS法测定人血浆中的福多司坦,并用于人体药动学研究。血浆经甲醇沉淀蛋白,采用氰基柱,对乙酰氨基酚为内标,甲醇-水(80:20)为流动相,ESI源负离子检测方式,多反应监测,选择离子反应为m/z 178.0→90.6(福多司坦)和m/z 149.9→106.7(内标)。福多司坦在0.2～20μg/ml浓度范围内线性关系良好,定量限为0.2μg/ml,提取回收率为87.2%～97.6%,日内及日间RSD均小于15%。
A LC-MS/MS method was established for the determination of fudosteine in human plasma. The pharmacokinetics in human was also investigated. The samples were treated with methanol, and paracetamol was used as internal standard. The CN column was used, with the mobile phase of methanol-water （80 ：20）. The detection was carried out by means of electrospray ionization mass spectrometry in negative ion mode. Multiple reaction monitoring （MRM） mode was used with the transitions of m/z 178.0→90.6 and m/z 149.9→106.7 for fudosteine and internal standard, respectively. The calibration curve of fudosteine was linear in the concentration range of 0.2 - 20μg/ml. The limit of quantification was 0.2 μg/ml. The extraction recovery of fudosteine was 87.2 % - 97.6 %. The RSDs of intra- and inter-day were less than 15 %.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2009年第5期365-367,共3页
Chinese Journal of Pharmaceuticals
作者简介
卢姗（1984-），女，硕士研究生，专业方向：药物分析。
通信联系人：李发美（1947-），女，教授，博士生导师，从事药物分析学研究。Tel：024．23986289 E-mail：lifamei@syphuedu．cn
