摘要
[目的]介绍一种适合于富含多糖、多酚植物材料的RNA提取方法。[方法]采用改进的异硫氢酸胍法提取植物材料的RNA，用紫外分光光度计测定其浓度和纯度，并通过RT-PCR进一步检测其质量。[结果]提取总RNA中的28、18、5s条带清晰，比例适中，OD260nm/OD280nm。为1．8—2．0。以提取的总RNA反转录合成的cDNA为模板，能扩增出预期长度的片段，无非特异性产物。[结论]该方法简便易行，成本较低，适合于富舍多糖、多酚植物材料的RNA提取。
[Objective] The study aimed to introduce a kind of method on extracting RNA from plant materials rich in polysaccharide and polyphenol.
[Method] The improved guanidine isothiocyanate method was used to extract RNA from plant, and the purity was used to determine with UV spectrophotometer. And its quality was further determined through RT-PCR. [Result] The 28, 18 and 5 S bands in the RNA extracted had clear bands and proper proportion, with OD260nm/280nm. Taking the synthesized cDNA of the extracted total RNA though reverse transcription as template, gene
sequence with expected could be amplified and non-specific products were not seen. [Conclusion] The method was simple, convenient and low cost. It
was suitable for extracting RNA from plant materials rich in polysaccharide and polyphenol.
出处
《安徽农业科学》
CAS
北大核心
2009年第12期5383-5383,5417,共2页
Journal of Anhui Agricultural Sciences
基金
河南省科技厅2007年立项课题(072300450330)资助
关键词
多糖
多酚
RNA提取
Polysaccharide
Polyphenol
RNA exllaction
作者简介
王文锋（1976-），女，河南长垣人，硕士，讲师，从事分子生物学科研与教学工作。
