摘要
文章所采用的多分蘖矮秆突变体为htd1-2(high-tillering dwarf 1-2),是野生型籼稻品种9311经350Gy的60Co-γ射线辐射处理后产生的后代中选育出来的稳定多分蘖矮秆突变体。遗传分析表明,突变体htd1-2多分蘖矮秆性状是由一对隐性核基因的突变造成的。文章利用简单重复序列(Simple sequence repeat,SSR)、酶切扩增多态性序列(Cleaved amplified polymorphic sequence,CAPS)和衍生型CAPS(derived CAPS,dCAPS)等分子标记的方法,最终将多分蘖矮秆基因HIGH-TILLERING DWARF1-2(HTD1-2)定位在水稻第4号染色体116 kb的物理区间内。在该物理区间内有一个已经克隆的控制水稻分蘖的基因HIGH-TILLERING DWARF1(HTD1),经过测序比对和dCAPS特异性分析,认为HTD1就是HTD1-2基因。尽管突变体htd1与突变体htd1-2是等位基因的不同位点发生突变,但是由于遗传背景的不同,两者表型并不完全相同。此外,通过去除分蘖芽的实验证明了突变体htd1-2的矮化部分是由于分蘖过多造成的。
Tillering is one of the most important agronomic traits of rice. In order to explore the molecular mechanism of rice filleting, a high-tillering dwarf 1-2 （htd1-2） mutant was isolated from the offspring of the indica rice （Oryza sativa L. ssp. indica） variety 9311 treated with 350Gy ^60Co γ-radiation. Genetic analysis showed that both high filleting and dwarf phenotypes of htd1-2 were controlled by a single recessive nuclear gene. By means of molecular marker technique, the HIGH-TILLERING DWARF1-2（HTD1-2） gene was mapped between two CAPS markers A6 and E2 on chromosome 4 within 116 kilobases. Within this region, there is a cloned gene HIGH-TILLERING DWARFI（HTD1）, which controls rice filleting. By comparing sequences of HTD1 between htd1-2 and 9311, in combination with the results from dCAPS analysis,we believed that HTD1 is an orthologue of the gene HTD1-2 .Because of different genetic backgrounds, htdl and htd1-2 have different phenotypes although they are the allelic mutants. Furthermore, removal of axillary buds proved that dwarfism of htd1-2 mutant is partly attributed to its excessive tillers.
出处
《遗传》
CAS
CSCD
北大核心
2009年第5期531-539,共9页
Hereditas（Beijing)
基金
教育部留学人员回国启动基金项目(编号:010144)
辽宁省百千万人才工程基金(编号:2008921057)资助
关键词
水稻
多分蘖
矮化
分子标记
HTD1基因
rice （Oryza sativa L.）
high-tillering
dwarfism
molecular marker
HTD1gene
作者简介
江海湃（1983-），男，硕士研究生，研究方向：作物生理学及分子生物学。Tel：024—88455679；E—mail：haipaijiang@hotmail.com
张淑英（1977-），女，博士，研究方向：作物生理学及分子生物学。Tel：010-64870491；E-mail：syzhang@genetics．ac．cn
通讯作者：王术（1968-），男，博士，副教授，研究方向：作物生理、品种改良及分子遗传学。Tel：024-88487135；E-mail：wangshu_sl@yahoo．com
