摘要
背景:利用先进的制造技术和各种生物材料可构造具有良好生物相容性、可降解性与一定外形及具有合理三维内部结构的支架,这种崭新的医学与工程技术相结合的方法,越来越多地应用到大段骨缺损研究中。目的:应用快速成形技术和冷冻干燥工艺制备多孔β-磷酸三钙,评价其结构性能并分析结构影响因素。设计、时间及地点:对比观察实验,于2008-05/08在上海大学快速制造中心实验室完成。材料:β-磷酸三钙浆料由上海组织工程研究与发展中心提供。方法:应用CAD软件设计具有圆柱形内腔的组合式负型,将负型三维CAD数据进行快速成形前处理后导入到FDM成形机加工,获得负型的实物模型。负型组装后,充填β-磷酸三钙浆料,经冷冻、干燥及高温焙烧制备最终的生物陶瓷支架。主要观察指标:①应用X射线衍射技术进行物相分析。②扫描电镜观察不同浓度浆料和不同预冻温度制备支架截面的微观形貌。③计算不同浓度浆料和不同预冻温度制备支架的孔隙率。结果:①X射线衍射曲线显示制备β-磷酸三钙支架与标准β-磷酸三钙的特征衍射峰位置基本一致。②扫描电镜显示支架内部的孔隙分布沿径向呈梯度结构,边缘地带孔径为10～30μm,内部孔径为100～300μm。③浆料质量浓度低,支架孔隙小、分布均匀、孔隙率高;预冻温度低,支架孔隙小。结论:通过冷冻干燥技术结合快速成形技术可以得到具备精确外形和良好孔隙结构的骨支架。
BACKGROUND： Innovative scaffolds with good biocompatibility, degradation, and three-dimensional internal structure could be prepared based on advanced manufacture techniques and various biomaterials, which have been frequently used to treat bone defect. OBJECTIVE： To evaluate the structural perlormance and analyze the structural impact factor of the .β-tricalcium phosphate （ β-TCP） scaffolds prepared based on rapid prototyping and freeze drying techniques. DESIGN, TIME AND SETTING： A contrast study was performed at the Rapid Manufacturing Engineering Center of Shanghai University from May to August in 2008. MATERIALS： β-TCP slurry was provided by Shanghai Tissue Engineering Research and Development Center. METHODS： The combined negative mold with cylindrical cavity was designed by CAD software. The physical models were fabricated by FDM machine. The bioceramic scaffolds were prepared by the process assembly of the combined negative molds, filling of β-TCP slurry, freeze drying and high-temperature calcination. MAIN OUTCOME MEASURES： （1） The materials component of the scaffolds was analyzed by X-ray diffraction technique. （2） The 3D structure of the scaffolds was inspected by scanning electron microscope.（3） The porosity of the scaffolds was calculated. RESULTS： （1） The diffraction peak position of the ,β-TCP scaffolds was identical with the standard β-TCP. （2） The scanning electron microscopy indicated that porosity was gradient-distributed along the radial. The pore size of edge region was 10- 30 μ m and the pore size of central region was 100-300 μ m. （3） The slurry concentration and pre-freezing temperature were structural impact factors of scaffolds. With the slurry concentration decreasing, the increasing water ratio resulted in higher porosity, and the pore distribution became more uniform. With the pre-freezing temperature decreasing, the smaller ice crystal resulted in the smaller pore size. CONCLUSION： The rapid prototyping combining with freeze drying technique can fabricate bone scaffolds with precise shape and good pore structure.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第16期3075-3078,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
上海市创新基金(09YZ34)
上海市重点学科开放基金(Y0102)~~
作者简介
林柳兰，女，1974年生，湖北省武汉市人，汉族，2004年华中科技大学毕业，工学博士，副研究员，主要从事快速成形和仿生制造研究。Linliulan@stafr．shuedu．cn
