摘要
碱量法是水产行业测定壳聚糖脱乙酰度的标准方法,应用广泛。该方法采用甲基橙-苯胺蓝指示剂,在测定低脱乙酰度壳聚糖时终点颜色变化不明显,重现性差,误差较大;且苯胺蓝有毒,易造成环境污染和人体危害。研究了用于碱量法测定的不同种类指示剂,结果表明:甲基橙-亚甲基蓝(1:1)混合指示剂终点颜色变化明显,准确性和重现性好,可以作为测定壳聚糖不同脱乙酰度的通用指示剂,解决了目前碱量法存在的通用性和毒害问题。
Alkalimetry is standard method of determining degree of deacetylation of chitosan generally applied in aquaculture and other industry. The method adopts helianthine-triphenylrosaniline as indicator, which endpoint color is difficult to observe, making poor reproducibility and wide error when low degree of deacetylation of chitosan is determined, and triphenylrosaniline has toxic, causing environmental pollution and harm to human health. In this paper, we have studied different types of indicators which can be used in alkalimetry, the results suggested that helianthine-triphenylrosanUine（1：1） mixed indicator can instruct color changes of titration end significantly, the accuracy and reproducible are good. This indicator is likely to become common indicator of determining different degree of deacetylation of chitosan, which can resolve current common and poison problem of alkalimetry.
出处
《食品科技》
CAS
北大核心
2009年第4期262-265,275,共5页
Food Science and Technology
基金
广东省科技计划项目(2006B37001006
2007B010600039)
关键词
碱量法
壳聚糖
脱乙酰度
指示剂
alkalimetry
chitosan
degree of deacetylation
indicator
作者简介
邬思辉（1984-），男，硕士研究生，研究方向为药用辅料和药物制剂。
通讯作者
