摘要
光镜观察发现,斑鳜肝脏浆膜极薄,厚度为(1.91±0.89)μm,边缘肝细胞排列紧密,肝小叶极不明显,肝细胞聚集成团,不能分辨出肝细胞索,肝血窦特别丰富,相互交织成网络状,可见枯否氏细胞,无典型汇管区,胆小管非常发达,呈环状或交织成网.电镜观察发现,斑鳜肝细胞具有明显的双态现象,可分为电子密度低的亮细胞和电子密度高的暗细胞.亮细胞多角形或形状不规则,异染色质较少,胞质内粗面型内质网发达,有成层分布现象.暗细胞多角形或卵圆形,异染色质较多,胞质内脂滴特别丰富.两种细胞胞质内线粒体数量众多,可见内含结晶样物质的次级溶酶体,核糖体丰富,糖原分散或聚集成小颗粒状,高尔基复合体靠近狄氏间隙和胆小管区域分布,肝细胞近狄氏间隙和胆小管面由细胞质突起形成丰富微绒毛,说明肝细胞的物质代谢、吸收和分泌功能非常旺盛.
Under light microscope, the chorion of the liver of Siniperca scherzeri is thin, thickness （1.91±0.89）μm and hepatocytes near the edge line tightly. The hepatic lobule is not obvious. The hepatoeytes get together into masses and the hepatocyte cords cannot be differentiated. The hepatic sinusoids are woven into net-shaped and the Kupffer, s cell is seen. The portal area is not typical. The bile eanalieuluses which show ring-shaped or are woven into nets.are very developed. Under electron microscope, The hepa- tocytes of Siniperca scherzeri show a distinct dimorphism and 2 types are identified based on their electron density, i. e. light cells and dark cells. The light cells whose rough endoplasmic reticulum is very developed show multiangle-shaped or irregular and have less heterochromatin, while the dark cells whose lipid droplets are especially plentiful show multiangle-shaped or oval and have more heterochromatin. In the 2 types of hepatocytes, there are abundant mitochondria and ribosomes, secondary lysosomes including crystal substance are seen, glycogen is scattered or gets together into little grains, Golgi complexes are near Disse,s space and bile canaliculus and there are very plentiful finger microvilli from hepatocytes in Disse,s space and bile canaliculus. All of these shows that the hepatocytes are very active in substance metabolizing, absorbing and secreting.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第2期43-48,共6页
Journal of Southwest China Normal University(Natural Science Edition)
基金
重庆市科委攻关资助项目(CSTC,2007AC1043)
关键词
斑鳜
肝脏
组织学
超微结构
Siniperca scherzeri
liver
histology
ultrastructure
作者简介
熊洪林（1967-），男，四川广安人，副教授，硕士，主要从事动物学和生态学研究．
通讯作者：王志坚．
