摘要
本研究针对血清在细胞库构建中的基础性作用,以中国农业科学院北京畜牧兽医研究所的20枚京星矮脚鸡的7日龄蛋胚为试验材料,利用2种不同的胎牛血清,采用贴壁培养方法,对细胞进行培养、传代及冻存。观察细胞原代及传代后的生长个体数、生长瓶数,细胞形态、冻存前形态及细胞生长曲线。结果表明,不同血清对细胞培养有一定的影响,血清B培养的细胞各方面都优于血清A培养的细胞。本研究结果为细胞体外培养中的血清选择提供了依据。
In order to study the basic effect of different serum on the construction of cell line, fibroblast cells were isolated from 20 seven-day old embryo of short-feet chicken and cultured, passaged and frozen via the method of adhere culture using 2 kind of FBS. The cell number, flask number, morphology and cell growth curve were observed. The results showed that various serum had significant effect on cell culture, and the cell characteristics were better when cultured with serum B than that with serum A. The present study provide the reference to the choice of serum on cell culture.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第4期96-99,共4页
China Animal Husbandry & Veterinary Medicine
基金
国家“863”计划资助项目(2007AA10Z170)
关键词
血清
动物细胞
体外培养
serum
animal fibroblasts
in vitro culture
作者简介
吴宏梅（1980-），女，陕西人，博士生，主要从事细胞与分子生物学研究。
通信作者：关伟军，男，教授，博导。Tel：010—62815992；Fax010—62895371；E—mail：wjguan86@iascaas．net．cn.
通信作者：马月辉，男，研究员，博导。Tel：010-62813463；Fax010-62895351；E-mail：Yuehui．ma@263．net
