摘要
目的：主要探索Ｔ－２毒素对体外培养软骨细胞致损临界值以及硒保护作用。方法：应用单层培养传代软骨细胞，用生化方法检测软骨细胞ＤＮＡ量和蛋白量，透射电子显微镜观察软骨细胞超微结构。结果：（１）Ｔ－２毒素在０．００１～０．００５ｍｇ／Ｌ培养液中（以下相同）剂量范围内，随浓度增加，Ｔ－２毒素对软骨细胞ＤＮＡ合成和细胞分裂增殖影响逐渐增大，加硒后该影响有所减弱，但仍不能阻止损伤的发生；（２）Ｔ－２毒素在０．０００５ｍｇ／Ｌ时，对软骨细胞的影响甚微；Ｔ－２毒素在０．００１ｍｇ／Ｌ和０．００５ｍｇ／Ｌ时，细胞损伤明显，与对照组比较有显著差别（Ｐ＜０．００１）。
In the present investigation,we observed the critical value of the damage of T2 toxin to chondrocytes and the protection of Se.The amounts of DNA and protein in the singlelayer cultured chondrocytes were assessed by the biochemical methods and the transmission electron microscope was utilized to observe the ultrastructure of chondrocytes.The results showed:(1) T2 toxin could restrain both the DNA synthesis and the cell proliferation of chondrocytes in the media containing different concentrations of T2 toxin (0.001￣0.005mg/L).The effect of T2 toxin on the chondrocyte damage was increased with the concentration of T2 toxin in the given does.When Se was added into the media,the chondrocyte damage by T2 toxin was decreased,but can not prevented.(2)T2 toxin led to few effects on chondrocytes as its concentration was 0.0005mg/L.It caused evident damage to chondrocytes when its concentration was 0.001mg/L and 0.005mg/L and the cell damage was significantly different from that in the control group (P<0.001).
出处
《中国地方病学杂志》
CAS
CSCD
1998年第3期143-146,共4页
Chinese Jouranl of Endemiology
