摘要
目的：探讨急性期反应物α１酸性糖蛋白（ＡＧＰ）和α１抗胰酶蛋白（ＡＴ）对人皮肤成纤维细胞（ＨＤＦｓ）的保护作用。方法：用培养的人皮肤成纤维细胞作为观察对象，分为不同浓度绿脓杆菌脂多糖（ＬＰＳ）处理组，每个脂多糖处理组再分为不同浓度ＡＧＰ或ＡＴ处理的亚组，用半自动细胞溶解测试法计算残存细胞，并计算绿脓杆菌ＬＰＳ的３０％细胞致死量（ＬＤ３０）。结果：在存在ＬＰＳ的环境中，ＨＤＦｓ存活数量减少，其存活数与ＬＰＳ剂量呈负相关；ＡＧＰ和ＡＴ均能提高ＨＤＦｓ的存活率。结论：绿脓杆菌ＬＰＳ对ＨＤＦｓ有直接的毒性效应（抑制其增殖，甚至致死）。
Objective: To test the hypothesis that acute phase reactants(APRs), such as α1acid glycoprotein (AGP) and α1antitrypsin (AT), provide important protection to human dermal fibroblast(HDFs) against further damage during tissue injury or infection. Methods: HDFs were cultured with DMEM plus 10% BCS at 37℃ in a 5% CO2 atmosphere. Different doses of lipopolysaccharide (LPS )from Pseudomonas aeruginosa and/or AGP (or AT) were supplemented. After 24 h, the semiautomated cell lytic assay was used to determine the survival condition of the HDFs. Results: The LPS from Pseudomonas aeruginosa showed cytotoxicities on HDFs. With the increase of LPS dosage in culture medium, the HDFs cell number decreased. Regression analysis showed a correlation coefficient of -0.892 (P<0.001) for the LPS concentration vs HDFs cell numbers. Both AGP and AT increased LD_30 of LPS on HDFs in a dosedependent fashion. Conclusion: (1) HDFs are susceptible to the directive toxicity of LPS from Pseudomonas aeruginosa; (2) Both AGP and AT are able to reduce the toxicity of LPS and/or increase the tolerance of HDFs to LPS.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1998年第S1期32-34,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
国家自然科学基金委员会杰出人才基金
上海市卫生系统百名跨世纪优秀学科带头人培养计划资助
