摘要
目的:利用ISSR分子标记技术初步检测和分析中国云南和内蒙地区毒品原植物大麻的遗传多样性。方法:用CTAR法提取大麻基因组DNA,设计10个ISSR引物,扩增产物采用6%中性聚丙烯酰胺凝胶电泳-硝酸银染色法检测,根据出现的条带数目和片段大小等分析大麻的多样性。结果:从10个ISSR引物中筛选出的4个引物用于2个地区的大麻基因组DNA扩增,PCR产物可以检测到51条重复性较好、带型清晰的DNA片段,其多态性总体比率为78.43%。云南地区和内蒙地区大麻样品可分别获得43和33条带,其中多态性条带分别为33条(76.74%)和21条(63.64%)。结论:ISSR分子标记技术揭示了大麻具有较高的遗传多样性,对于鉴别犯罪现场大麻检材的产地及种属来源具有一定的价值。
Objective:The aim of is to investigate genetic diversity of hemp cultivars from Yunnan Province and Inner Mongolia autonomous regions in China by using inter-simple sequence repeat(ISSR) molecular markers.Method:The genomic DNA of hemp cultivars was extracted by CTAB methods.We designed 10 ISSR primers to amplify,and amplified products were detected by 6% polyacrylamide gel electrophoresis followed by silver staining method.Genetic diversity of hemp cultivars was analyzed according to the number of bands and fragments size.Result:10 molecular markers of ISSR were used for detecting on hemp cultivars,of which 4 markers obtained good diversity and clear bands were selected for PCR.The amplification products of 4 ISSR primers could generate totally 51 bands,and the polymorphism rate was 78.43%.43 and 33 bands of hemp cultivars from two regions were generated by using 4 ISSR primers,and the polymorphic rate was 76.74% and 63.64%.Conclusion:Molecular markers of ISSR methods would be better to show a high genetic diversity of hemp cultivars,which could be provide new insight for identifying types and sources of drug plants.
出处
《生物技术》
CAS
CSCD
北大核心
2012年第4期50-52,共3页
Biotechnology
基金
河南省新乡医学院高学历人才启动基金项目(编号:2005)资助
关键词
法医学
大麻
简单重复序列间扩增
遗传多样性
forensic medicine
hemp cultivars
inter-simple sequence repeat
genetic diversity
