摘要
实验室分离到1株对香蕉枯萎病菌(Fusarium oxysporum f.sp.cubens)有明显抑制作用的菌种,经18SrDNA鉴定为木霉属,命名为Trichoderma sp.G2。采用固体和液体培养法对菌株G2抑制香蕉枯萎病菌的生长进行了初步研究。结果表明,在对峙试验的平板上,7d香蕉枯萎病菌完全被G2的短绒状菌丝及分生孢子梗丛覆盖;在拮抗平板上,G2发酵液的平均抑制率约为90.4%,挥发性物质平均抑制率达68.3%,可见挥发性物质对抑制病原菌的生长起了重要的作用。在浓度为1.0×107cfu·mL-1香蕉枯萎病菌液体培养中,按体积比为20%接种G2,10d后镜检发现病原菌的菌丝及孢子大部分出现融解,表明菌株G2对香蕉枯萎病菌有明显的拮抗作用。
A fungal strain, designated as G2, with remarkable ability of effective antagonist against Fusarium oxysporum f. sp. cubens, was isolated and identified as Trichoderma sp. based upon 18S rDNA gene analysis. With solid and liquid cultures, the inhibitive efficacy to the growth of Fusarium oxysporum f. sp. cubens was primarily studied. The experimental results showed that the cells of Fusarium oxysporum f. sp. cubens were completely covered by short fiber-like hyphace and spore stem of G2 within 7 days in the dual culture plate, and in the antagonist plate, the average rate of inhibitory by the culture solution of G2 was about 90.4%, the average rate of the inhibitory by volatile substance reached 68.3%. After 10 days＇ incubation with 20% （v/v） fungal strain G2, the melt of the pathogenic mycel and spore were observed in the liquid culture containing 1.0×10^7 cfu. L^-1 Fusarium oxysporum f. sp. cubens, suggesting that the strain G2 can strongly inhibit the growth of Fusarium oxysporum f. sp. cubens.
出处
《果树学报》
CAS
CSCD
北大核心
2009年第2期186-189,F0003,共5页
Journal of Fruit Science
关键词
香蕉
木霉菌
香蕉枯萎病菌
抑制作用
Banana
Trichoderma sp.
Fusarium oxysporum f. sp. cubens
Antagonism
作者简介
钟小燕，女，助理工程师。Tel：0750-2330986，E-mail：zhongxy_pico@126．com
