摘要
以金正日球根海棠（Begonia tuberhybrieda Vosa）的叶片为外植体进行组织培养的诱导，以Ms培养基为基本培养基，并附加不同浓度的细胞分裂素6-BA以及生长素NAA或2，4-D进行组织培养和植株再生。经实验筛选出最适合诱导愈伤组织的培养基是Ms＋6-BA1．0～2．0＋NAA0．1～0．5；脱分化的最佳激素组合是培养基Ms＋6-BA1．0＋NAA0．5。
eaves of Kim Jong-il Begonia tuberhybrieda Vosa were used as explants to conduct tissue culture induction. Ms medium was basic medium,adding with different concentrations of cytokinin 6-BA and NAA or 2 ,4-D to carry on plant tissue culture and regeneration. The experiment selected the most suitable callus induction of the medium was MS＋BA1.0-2.0＋NAA0.1-0.5;dedifferentiation hormone combination was the best medium MS＋BA1.0＋NAA0.5.
出处
《黑龙江农业科学》
2009年第2期17-19,共3页
Heilongjiang Agricultural Sciences
关键词
球根海棠
组织培养
诱导
Begonia tuberhybrieda Vosa
tissue culture
induction
作者简介
张凤生（1977-），男，黑龙江绥化市人，学士，农艺师从事园艺类组织培养研究。Tel：13936322142;0451-84302560。
