摘要
目的：建立从人类肺组织中提取并检测多环芳烃（PAHs）的方法。方法：人肺组织经超声萃取-SPE净化，提取液浓缩过滤后，经高效液相色谱分析。结果：各PAHs标准曲线的线性范围在1．2～392．2ng／ml之间，检出限在0．6—4．2ng之间，基质加标回收率在68．6％～103．7％之间，相对标准偏差在10％以内。结论：该方法线性关系良好，分离效果好，灵敏度高，能满足肺组织中PAHs的检测。
Objective：To establish a method of extracting and detecting polycyclic aromatic hydrocarbons （PAHs）in human lung tissue. Methods：The human lung tissues were extracted by ultrasonic wave and cleaned up by solid phase extraction. After being concentrated and filtrated, the PAHs were analyzed by HPLC. Results：The calibration curves were linear within the range of 1.2 - 392. 2 ng/ml, the detection limits ranged from 0.6 to 4. 2 ng, the recovery ranged from 68.6% to 103.7% with the RSD less than 10%. Conclusion：The method has good linear relation and good effect of separation. It is highly sensitive and can meet the demand of human lung tissue analysis.
出处
《中国卫生检验杂志》
CAS
2009年第2期331-332,384,共3页
Chinese Journal of Health Laboratory Technology
基金
青岛科技发展指导计划资助项目(2000-178-61)
关键词
高效液相色谱
超声萃取
固相萃取
多环芳烃
人肺组织
High performance liquid chromatography
Ultrasonic wave extraction
Solid phase extraction
Polycyclic aromatichydrocarbon
Human lung tissue
作者简介
张涛（1982-），男，在读硕士，主要从事临床生物化学研究。
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