摘要
目的探讨茶多酚干预后链脲佐菌素(Streptozotocin,STZ)诱导的糖尿病大鼠晶状体中线粒体锰超氧化物岐化酶(MnSOD)的mRNA表达的变化,从分子水平探讨糖尿病性白内障发病中氧化应激机制的作用以及茶多酚对糖尿病性白内障预防治疗的作用和机制。方法将90只大鼠随机分为3组,分别为正常组、STZ诱导糖尿病组(STZ-DM)和茶多酚(Tea Polyphenols,TP)干预糖尿病组(TP-DM)。TP-DM在建立糖尿病模型的同时,应用茶多酚进行干预治疗,采用RT-PCR检测各组大鼠晶状体中MnSOD mRNA的表达量,研究不同病程糖尿病大鼠晶状体MnSOD表达的变化及茶多酚干预的影响。结果正常组大鼠晶状体中MnSOD的mRNA表达量随时间变化差异无统计学意义(F=1.667,P>0.05),与β-actin表达量比值为0.723±0.031。STZ-DM大鼠晶状体中MnSOD mRNA2周时表达量最高,之后2者表达均下降,但4、8周时仍然高于正常组,至12周时才降至正常水平;不同病程各组大鼠晶状体MnSOD mRNA的表达量差异有统计学意义(F=1554.47,P<0.01)、(F=7215.93,P<0.01)。TP-DM组大鼠晶状体中的MnSOD mRNA表达在2、4、8、12周时均高于STZ-DM组(F=261.587,P<0.01),但随时间变化差异无统计学意义(F=53.32,P>0.05)。结论茶多酚可以上调糖尿病大鼠晶状体中MnSOD mRNA的表达并使其维持在较高水平,进而影响晶状体中活性氧的清除,阻止或者延缓晶状体的混浊,显著延缓糖尿病性白内障的发生。
OBJECTIVE To study the expression changes of MnSOD （manganese superoxide dismutase, MnSOD） in lens of diabetic rats induced by STZ（Streptozotocin, STZ） after （Tea Polyphenols,TP）TP＇s intervention,to discuss the molecular mechanism of oxidative stress in diabetic cataract and to study the effect and mechanism of prevention and cure of diabetic cataract by TP. METHODS Ninety rats were randomly divided into normal group, STZ induced diabetes mellitus group （STZ-DM）and TP intervened diabetes mellitus group （TP-DM）. While establishing rat model of diabetes mellitus,TP was given to TP-DM group to intervene simultaneously, and RT-PCR was used to detect the expression of MnSOD mRNA of rat lens in each group. The biochemical indicators and MnSOD expression of diabetic rat lenses in different courses and the intervention effects of TP were studied. RESULTS There were no significant differences in normal group＇s expression of MnSOD mRNA （F=1.667,P〉0.05）, expression ratio with β-actin was 0.723± 0.031 ;The expression of MnSOD mRNA gradually decreased in STZ-DM group. There were significant differences in different later decreased, at 4, groups＇ MnSOD mRNA （F=1554.47,P〈O.O1）,（F=7215.93,P〈0.O1）,highest at 2 weeks, 8 weeks still higher than normal group; The expression of MnSOD mRNA increased and retained at a relevantly high level. In TP - DM group ,there were no significant differences in TP-DM group＇s expression of MnSOD（F=53.32,P〉 0.05）. CONCLUSIONS The expression of MnSOD mRNA gradually decreased in STZ-DM group, TP could up-regulate the level of the expression of MnSOD mRNA in diabetic rat lens and maintain it at a relatively high level. Accordingly, it could influence the elimination of ROS in lens, prevent or delay the opacity of lens, and significantly delay the occurrence of diabetic cataract.
出处
《中国中医眼科杂志》
2009年第1期1-4,共4页
China Journal of Chinese Ophthalmology
基金
山东省技攻关计划(GG3WZ02036)
作者简介
通讯作者：解孝峰，E—mail：yankeboshi@126．com
