摘要
目的探讨成人初治急性非淋巴细胞白血病（ＡＮＬＬ）Ｐ糖蛋白（Ｐｇｐ）表达及罗丹明１２３排出活性（Ｒ１２３Ｅ）与预后的关系。方法流式细胞术检测ＡＮＬＬ白血病细胞Ｒ１２３Ｅ及Ｐｇｐ表达。结果Ｒ１２３Ｅ＋率及Ｐｇｐ＋率分别为３９５％及３４２％。Ｍ３的Ｒ１２３Ｅ＋率（０／６）明显低于其他亚型（４３８％）（Ｐ＜００５）。Ｐｇｐ表达与活性存在明显相关性（Ｐ＜０００１），７８９％的患者表达与活性一致（１０例Ｐｇｐ＋Ｅ＋，２０例Ｐｇｐ－Ｅ－）；２１１％存在表达与活性分离（３例Ｐｇｐ＋Ｅ－，５例Ｐｇｐ－Ｅ＋），Ｐｇｐ－Ｅ＋提示非Ｐｇｐ介导的排出活性在ＡＮＬＬ耐药中可能具有重要作用。Ｒ１２３Ｅ＋完全缓解（ＣＲ）率（２／１１，１８２％）及Ｐｇｐ＋者的ＣＲ率（２／１０，２０％），明显低于Ｒ１２３Ｅ－的９２９％（１３／１４）及Ｐｇｐ－的８６７％（１３／１５）（Ｐ分别＜００１及＜０００１）。
Objective To explore the relationship between P glycoprotein (P gp) activity and expression and the prognosis of acute nonlymphoblastic leukemia (ANLL). Methods P gp and rhodamine 123 (R123) efflux were determined simultaneously in 38 previously untreated adult ANLL patients with flow cytometry. Results The positivity of P gp expression and R123 efflux was 34.2% and 39.5%, respectively. The positivity of R123 efflux (R123 E +) was lower in M 3 (0/6) than that (43 8%) in non M 3 ( P <0.05). P gp expression was significantly correlated with R123 efflux ( P <0.001), the conformity rate was 78.9% (30/38, 10 P gp +E +, 20 P gp -E -). But discordance between the two methods existed in 8 (21.1%) cases (5E +P gp -, 3E -P gp +). E +P gp - suggested that non classic multidrug resistance (MDR1) mediated efflux system might exist in ANLL. The remission rates of P gp + (2/10, 20%) and E + (2/11, 18.2%) were significantly lower than those of P gp -(13/15, 86 7%) and E - (13/14, 92.9%) ( P <0.01 and <0.001, respectively). Conclusion It was suggested that both P gp expression and activity had prognostic significance in ANLL.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1998年第4期262-262,共1页
Chinese Journal of Internal Medicine
基金
江苏省科委和江苏省卫生厅基金
关键词
白血病
非淋巴细胞
急性
P糖蛋白
若丹明
Leukemia, nonlymphocytic, acute Flow cytometry P glycoprotcin Rhodamines
