摘要
采用人工培养箱技术,研究ETH、6-BA及其复配处理对大豆幼苗的形态特征、生物量、生化成分、内源激素以及细胞组织结构的调节效应,为大豆芽菜的安全生产提供理论和技术支持。结果表明:不同处理之间大豆幼苗形态存在差异(P<0.05)。ETH、6-BA及复配处理,均抑制了大豆幼苗的根和下胚轴的生长,促进下胚轴的增粗,三者处理后的根长分别比对照减少了24.1%(P<0.01),44.7%(P<0.01)和51.7%(P<0.01);下胚轴长分别比对照减少39.2%(P<0.01),4.1%(P>0.05)和26.2%(P<0.05);下胚轴粗分别比对照增加18.5%(P<0.01),9.2%(P<0.05)和32.3%(P<0.01)。大豆幼苗形态上的差异可以通过其下胚轴细胞组织结构的变化来解释,ETH促进了细胞的横向分裂与皮层细胞的扩大,促进下胚轴的增粗,抑制了细胞的径向分裂;6-BA促进了皮层细胞的横向分裂与扩大,抑制了细胞径向的伸长;ETH-6-BA复配处理促进了皮层薄壁细胞的分裂与扩大。不同处理之间大豆幼苗的维生素C含量存在差异(P<0.05);与对照相比,处理组大豆幼苗的GA含量降低,ABA含量增高(P<0.01)。
By the growth chamber method, the effects of ETH and 6- BA on the morphological features, biomass, chemical composition ,endogenesis hormones and cell tissue structure of soybean seedling were studied. In order to provide theory basis for soybean sprout production. The results showed that there were significant differences among the treatments in the morphological features （P 〈 0.01 ）. Compared with the control ,the growth of roots and hypocotyls was suppressed and the diameter of hypoeotyls was increased when the seedlings were treated by ETH,6-BA and ETH -6-BA. The root length were reduced by 24.1% （P 〈 0.01 ） ,44.7% （P 〈 0.01 ） and 51.7% （ P 〈 0.01 ）, respectively. The hypocotyls length reduced by 39.2% （ P 〈 0.01 ）, 4.1% （ P 〉 0.05 ） and 26.2% （ P 〈 0.05 ）, respectively. The hypocotyl diameter increased by 18.5 % （P 〈0.01 ） ,9.2% （P 〈0.05） ,32.3% （P 〈0.01 ） ,respectively. All of these can be explained by the variations of the cell tissue structure. ETH promotes the cellular transverse direction division and the cerebral cortex enlargement, depresses the radial direction division. 6- BA promotes the cerebral cortex cellular transverse direction and ambit division, while depresses the cell radial elongation. ETH - 6- BA promotes the division and enlargement of the cerebral cortex cell. There were significant differences among the treatments on vitamin C content （ P 〈 0.05 ）. Compared with the control, the GA content decreased and the ABA content increased in the five treatments（P 〈 0.01 ）.
出处
《大豆科学》
CAS
CSCD
北大核心
2009年第1期41-45,52,共6页
Soybean Science
关键词
大豆幼苗
ETH
6-BA
形态特征
生化成份
细胞组织结构
ETH and 6- BA
Morphological feature
Chemical composition
Endogenesis hormone
Cell tissue structure
作者简介
廖永霞（1985-），女，学士。研究方向为种子科学与工程。
通讯作者：康玉凡，博士，副教授。E-mail：yfkang@cau．edu．cn。
