摘要
目的了解2007-2008年间吉林省H3N2亚型流感病毒HA1基因的演变特征。方法采用MD-CK细胞分离培养流感病毒,提取病毒RNA,经逆转录和聚合酶链式反应扩增后测序。结果①2007-2008年吉林省流行的H3N2亚型流感毒株核苷酸同源性为98.9%～100%,与同期WHO推荐的疫苗株A/Wisconsin/67/05相比有10～14个氨基酸位点发生变化,并且在122位增加了一个糖基化位点;②长春市11月与12月分离的病毒株在进化树上处于不同的两个侧枝;③在同一流行高峰吉林省8个市州分离的流感病毒株在进化树上形成不同的两簇。结论2007-2008年吉林省流行的H3N2亚型流感毒株与WHO推荐的疫苗株A/Wisconsin/67/05相比已经发生一定变化;不同时间及市州流行的病毒株也有差异。
Objective To analyze the characteristics of HA1 gene of influenza H3N2 isolated from Jilin province during 2007 - 2008, and elucidate the relationship between the influenza epidemic and the variation of HA1 region of the virus. Methods The viruses were isolated and propagated by MDCK cell. RNAs were extracted and their HA1 genes were amplified by reverse transcription polymerase chain reaction （ RT - PCR）. The purified PCR products were sequenced. Results （1）The pandemic strains of influenza H3N2 isolated in Jilin province 2007 -2008 had 98. 9% - 100% identity of nueleotides. Compared with A/Wisconsin/67/05 vaccine strain recommended by WHO , 10 - 14 amino acid had changed, and there was one more glycosylation site at 122nd site. （2）The virds isolated in Changchun city in November and December located in two different branches of the phylogenetic tree. （3）The influenza strains of eight regions formed two clusters in the phylogenetic tree. Conclusion Compared with A/Wisconsin/67/05, The viruses of influenza H3N2 in Jilin province 2007 -2008 occur evident antigenic drift in HA1 region , and the influenza pandemic strains have some difference in different times and regions as well.
出处
《中国卫生工程学》
CAS
2009年第1期5-7,共3页
Chinese Journal of Public Health Engineering
作者简介
许爽（1978-），女，吉林长春人，硕士在读，主管技师，研究方向：病毒病原学。
通讯作者：谭岩（1956-），男，吉林长春人，博士生导师，主任医师，研究方向：免疫学及分子生物学。
