摘要
目的:采用GC法测定麝香壮骨膏中樟脑,薄荷脑,冰片,水杨酸甲酯的含量,以评价麝香壮骨膏的质量。方法:色谱条件为:毛细管柱PEG-20M,30m×0.32mm×0.25μm,程序升温:从100℃开始以5°C每分钟升到130°C,再以30°C每分钟升温到200°C,随后持续2min[2]。进样口温度:220°C,氮气:5mL/min,氢气流速:40mL/min;空气流速:450mL/min;检测器温度:240°C。结果:在选定的条件下樟脑,薄荷脑,冰片,水杨酸甲酯分别在0.02738mg/mL～0.43808mg/mL、0.04115mg/mL～0.6584mg/mL、0.03338mg/mL～0.5341mg/mL、0.04215mg/mL～0.6744mg/mL范围内与峰面积/内标面积呈良好的线性。平均回收率分别为98.37%,99.76%,98.80%,97.61%,RSD分别为1.7%,1.8%,0.96%,1.5%。结论:本试验为麝香壮骨膏的分析提供了一种准确可靠的检测方法。
AIM ： To study the method of determing camphor, menthol, synthetic bornel and the wintergreen oil contents in Shexiang Zhunggu Plaster. METHODS： Gas phase chromatogram was used to determine contents of camphor, menthol, synthetic bornel and the wintergreen oil in Shexiang Zhuanggu Plaster. The chromatogram con- dition consisted of the capillary vessel column（ PEG-20M, 30 m × 0.32 mm× 0.25μm）. The temperature progra- ming rose from 100 ℃ to 130 ℃ by 5 ℃/minute, then 30 ℃/minute to 200 ℃, lasting 2 minutes in the end. The temperature of the entrance of the capillary vessel column： at 220 ℃, temperature of the detector was 240 ℃ ; N2 ： 5 mL/minute, H2 ： 45 mL/minute, Air： 450 mL/minute ; sample quantity was 0.2 μL. RESULTS ： Under certain chromatogram condition, there was linear relation among the camphor, menthol, synthetic bornel and the winterg- reen oil and the peak area in the scope of 0. 027 38 mg/mL-0. 438 08 mg/mL, 0.04115 mg/mL-0.658 4 mg/mL, 0.033 38 mg/mL-0. 534 1 mg/mL, 0. 042 15 mg/mL-0. 674 4 mg/mL, respectively. The average recoveries were 98.37%, 99.76%, 98.80% and 97.61%, respectively. RSD were 1.7%, 1.8%, 0.96% and 1.5%, respec- tively. CONCLUSION： This experiment provides an accurate and reliable method to control the quality of Shex- iang Zhuanggu Plaster.
出处
《中成药》
CAS
CSCD
北大核心
2009年第2期231-234,共4页
Chinese Traditional Patent Medicine
关键词
麝香壮骨膏
樟脑
薄荷脑
冰片
水杨酸甲酯
GC
Shexiang Zhuanggu Plaster
camphor
menthol
synthetic bornel
wintergreen oil
GC
作者简介
刘翔（1970-），男，副主任药师，硕士，从事药品检验及质量标准研究。Tel：（0511）8616000-8202E-mail：liuxiang8941@163．com
