摘要
目的探讨高强度聚焦超声（HIFU）对兔肝肿瘤模型的肿瘤微血管密度（mircrovessel density，MVD）、血管内皮细胞生长因子（vascular endothelial growthfactor，VEGF）的影响。方法用30只新西兰白兔制成VX2细胞肝癌模型，分为对照组（5只）和HIFU消融组（25只），于术后即时、1d、3d、7d、16d取材，用免疫组织化学SABC法观察并测量肿瘤组织的MVD和VEGF的表达。结果HIFU消融后，肿瘤组织MVD和VEGF的表达较术前明显降低（P〈0.01）．但HIFU消融组内不同时间点间MVD及VEGF差异无统计学意义（P〉0.05）。结论HIFU消融可有效地破坏肿瘤组织的微血管，抑制血管形成并减少肿瘤组织的血供。
Objective To study the effect of high intensity focused ultrasound （HIFU） ablation on microvessel density（MVD） and vascular endothelial growth factor（VEGF） in rabbit hepatcocellular carcinoma （HCC） models. Methods The VX2 tumor cells were planted in the hepatic left central lobe on 30 rabbits. All animals were divided into two groups randomly： HIFU group（ n = 25） and control group（ n = 5）. The MVD and the expression of VEGF in the HCC was detected by immunohistochemical SABC methods in control group and 0, 1 ,3,7,16 d after the operation in HIFU group. Results The MVD and the expression of VEGF in the HCC tissue after HIFU treatment in the HIFU group were decreased significantly as compared with those in the control group （ P〈0.01 ）. However, there was no significant difference in MVD and the expression of VEGF in the HCC tissue after HIFU treatment in the HIFU group among the different time points （ P 〉 0.05）. Conclusions HIFU treatment can effectively destroy the micovessel, repress the neovascularization and reduce the blood supply of the HCC.
出处
《中华超声影像学杂志》
CSCD
北大核心
2009年第1期71-74,共4页
Chinese Journal of Ultrasonography
基金
基金项目：国家自然科学基金（30570489）
关键词
超声检查
超声
高强度聚焦
肝肿瘤
内皮生长因子
Ultrasonography
Ultrasound, high-intensity focused
Liver neoplasms
Endothelial growth
作者简介
通信作者：周晓东．Email：zhouxd@fmmu．edu．cn
