摘要
通过工业污水的急性毒性试验,统计多刺裸腹溞(Moina macrocopa)的死亡个数,用概率单位法得出污水对多刺裸腹溞24h、48h的半致死剂量(LC50)分别为17.68%和14.56%,污水属于极毒性。通过半致死剂量设定对照组和5个浓度组(1%、2%、4%、8%、16%),测定污水对多刺裸腹溞的心率、体长、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽-S-转移酶(GST)的影响。结果表明:随污水浓度的增加和时间的延长心率和体长均先升高后降低,SOD、CAT和GST3种酶活性总体呈现"低促高抑"的变化趋势,当污水浓度为1%时,SOD活性的变化大于CAT和GST,故SOD酶是机体抵制外界不良环境中重要的酶,为水质监测提供更为敏感的生物指标。
An acute toxicity test was conducted to assess the toxicity of an industrial wastewater to Moina macrocopa. The 24 h and 48 h semi-lethal （ LC50 ） for M. macrocopa were 17.68% and 14. 56%, respectively, indicating that the industrial wastewater was very toxic. Based on the LC50, six concentrations （0, 1% , 2% , 4% , 8% and 16% ） of the industrial wastewater were installed to study their effects on the heart rate, body length, and activities of superoxide dismutase （ SOD）, catalase （CAT） and glutathione S-transferase （GST） of M. macrocopa. The results showed that with increasing concentration of wastewater and with increasing time, the heart rate and body length of M. macrocopa increased first and decreased then; while the activities of GST, CAT and SOD had a trend of increasing at low concentration wastewater but decreasing at high concentration wastewater. When exposed to the wastewater, SOD activity had a larger variation than GST and CAT activities, suggesting that SOD could be an important enzyme for organisms to resist bad environment, and a sensitive bio-indieator for wastewater monitoring.
出处
《生态学杂志》
CAS
CSCD
北大核心
2009年第2期300-303,共4页
Chinese Journal of Ecology
基金
国家自然科学基金项目(30570194)
山西省青年科技基金资助项目(20051035)
关键词
多刺裸腹溞
心率
体长
抗氧化酶系
工业污水
Moina macrocopa
heart rate
body length
antioxidase
industrial wastewater.
作者简介
王茜，女，1978年生，讲师。主要从事环境毒理方面的研究，发表论文6篇。E—mail：wangqian@sxu．edu．cn.
通讯作者E-mail：lanwang@sxu．edu．cn
