摘要
目的：建立快速、简便、灵敏的蔬菜中五氯硝基苯残留的高效液相色谱检测方法。方法：蔬菜样品经丙酮/正己烷（10：90,v/v）均质提取,用浓硫酸净化,在XDB C8（150 mm×4.6 mm i.d.,5μm）色谱柱上,以甲醇/乙酸-乙酸铵溶液（10 mmol/L,pH4.5,70/30,v/v）为流动相,流速为1.0 ml/min,柱温为35℃,紫外检测波长为254 nm。结果：蔬菜中五氯硝基苯的平均回收率在83.2%～94.2%范围,RSD小于5.5%,线性范围为0.1～50.0 mg/L（r=0.9992）,检出限为0.02 mg/kg。结论：建立的方法用于蔬菜中五氯硝基苯残留的测定,具有操作简便、快速、准确的优点。
Objective：To establish a simple,accurate and sensitive method for the determination of pentachloronitrobenzene residue in vegetables by liquid chromatography with ultraviolet detection.Methods：After the sample was extracted with acetone/hexane（10：90,v/v） and cleaned up with H2SO4,a satisfactory separation was achieved on a Zorbax XDB C8 column（150 mm×4.6 mm i.d.,5 μm）using methanol/acetic acid-ammonium acetate（10 mmol/L,pH 4.5,70/30,v/v） as the mobile phase at a constant temperature of 35℃ and at a flow rate of 1.0 ml/min.Detection was performed on an ultraviolet detector at 254 nm.Results：The linear range was 0.1～50.0 mg/L and the limit of quantification was 0.02 mg/kg.The extraction recoveries were 83.2%～94.2% and the RSD less than 5.5%.Conclusion：This method is simple,rapid and accurate for the determination of pentachloronitrobenzene in vegetables.
出处
《中国卫生检验杂志》
CAS
2008年第12期2587-2588,2733,共3页
Chinese Journal of Health Laboratory Technology
作者简介
邬晨阳（1963-），男，大学本科，副主任技师，主要从事理化检验及研究工作。
