摘要
采用构建16SrDNA克隆文库的方法,对实验室保存的1株产毒塔玛亚历山大藻在不同时期的藻际细菌群落多样性进行了分析.限制性片段长度多态性(restrictionfragment length polymorphism,RFLP)结果表明,塔玛亚历山大藻藻际微生物的16SrDNA克隆文库中的克隆子总共可分为34种基因型,选取各谱型的代表克隆子测定其16SrDNA片段核苷酸序列,将所获得的序列与GenBank数据库进行BLAST比对,结果表明所有基因型分属于2个细菌类群:变形细菌门(Proteobacteria)和拟杆菌门(Bacteroidetes).在延滞期的藻培养液中,α-Proteobacteria占36.4%,β-Proteobacteria占9.1%,γ-Proteobacteria占27.3%,拟杆菌门(Bacteroidetes)占27.3%;在指数后期的培养液中,α-Proteobacteria占53.3%,β-Proteobacteria占13.3%,γ-Proteobacteria占6.7%,拟杆菌门(Bacteroidetes)占26.7%;在稳定期的培养液中,α-Proteobacteria占47.8%,β-Proteobacteria占8.7%,γ-Proteobacteria占21.7%,δ-Proteobacteria占4.3%,拟杆菌门(Bacteroidetes)占17.4%;其中有不少克隆子与已知序列同源性低于94%,表明塔玛亚历山大藻藻际环境中附着有新的未开发的微生物资源,这些细菌可能在微藻的生消过程中起着重要的调控作用,所以本研究结果在赤潮微生物调控中具有重要的理论意义和应用价值.
The composition of bacterial community in the Alexandrium tamarense culture was determined by analyzing the 16S rDNA clone libraries. 16S rRNA gene was amplified by using the total DNA extracted from the A. tamarense culture at three growth stages as templates.34 different restriction fragment length polymorphism（RFLP） patterns of the clones were obtained from the three libraries. Clones representing each RFLP patterns were sequenced and phylogenetic analysis revealed that the Proteobacteria and the Bacteroidetes group can be the dominant component in the phycosphere microflora. Results showed that in the lag phase, α-Proteobacteria（36.4%）, γ-Proteobacteria（27.3% ） and Bacteroidetes（27.3%） were the dominant group; in the late-exponential phase, the clones belonged to α-Proteobacteria （53.3%）, γ- Proteobacteria（13.3% ）,γ-Pmteobacteria（6.7% ）, and Bacteroidetes （26.7%）;and in the stationary phase, the community was comprised of α-Proteobacteria （47.8 % ）, β Proteobacteria （ 8.7 % ）, γ- Proteobacteria （ 21.7 % ）, δ- Proteobacteria （ 4.3 % ） and Bacteroidetes （ 17.4 % ）. The results also suggest that a part of the bacteria associated with A. tamarense are uncultured and novel species. And these bacteria may play a major role in regulating the processes of algal bloom initiation, maintenance and decline. So these results may provide us great academic and practical importance in controlling the algal blooms by microbes.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2009年第1期271-279,共9页
Environmental Science
基金
国家高技术研究发展计划(863)项目(2008AA09Z408)
长江学者和创新团队发展计划项目(40521003)
国家重点基础研究发展规划(973)项目(2006CB400605)
国家海洋局海洋生态系统与生物地球化学重点实验室开放基金项目(LMEB200601)
教育部博士点专项基金项目(20050384002)
中国博士后科学基金项目
作者简介
杨小茹（1980-），女，博士研究生，主要研究方向为环境微生物学，mail：xryang@xmu.edu.cn
通讯联系人，E-mail：wshwzh@xmu．edu．cn
