摘要
目的：探讨组织蛋白酶DalRNA转录和蛋白表达与卵巢癌发生、发展及预后的关系。方法：选取2006年1月～2007年12月卵巢癌29例，对照组为14例正常卵巢组织，RT-PCR法和WB法检测两组中组织蛋白酶D mRNA转录和蛋白表达水平。CD34免疫组化法进行卵巢癌微血管密度（microvessel density，MVD）检测，统计不同病理分级及临床分期卵巢癌组织蛋白酶D阳性表达和MVD的关系。结果：卵巢癌组组织蛋白酶D mRNA转录和蛋白阳性表达率为62．1％和67．0％，均显著高于埘照组（P〈0.01）。低分化卵巢痛组织蛋白酶D mRNA转录和蛋白阳性率为92．9％和100％，显著高于高巾分化标本（P〈0．01）。Ⅲ～Ⅳ期卵巢癌标本组织蛋白酶D mRNA转录和蛋白阳性率为91．7％和100％，显著高于Ⅰ～Ⅱ期标本（P〈0．01）。有淋巴结转移的卵巢癌组织蛋白酶D mRNA转录和蛋白阳性率为91．7％和100％，显著高于未转移标本（P〈0．01）。低分化、Ⅲ～Ⅳ期及转移卵巢癌中MVD分别为（45．6±3．6）、（47．8±4．1）和（46．6±3．9）个，分别显著高于高中分化、Ⅰ～Ⅱ期和未转移标本。组织蛋白酶D mRNA转录和蛋白阳性卵巢癌MVD分别为（35．3±4．1）个和（34．2±4．3）个，显著高于组织蛋白酶D mRNA转录和蛋白阴性卵巢癌标本。结论：卵巢癌中组织蛋白酶D转录及蛋白水平均高于正常卵巢组织，其表达与卵巢癌病理分级及临床分期显著相关。
Objective：To detect the level of protein expression and mRNA transcription of cathepsin D, study their relationship with the genesis, development and prognosis of ovarian cancer. Methods：29 speeimens of ovarian cromer from Jan, 2006 to Dec, 2007 were included in ovarian cancer group, while 14 specimens of normal ovary were included in eontrol group. The mRNA and protein expression of cathepsin D of both groups were detected by RT - PCR and Western blot. Microvessel density （MVD） was detennined by immunohistochemical stain with CD34 antibody. The relationship between MVD and positive prolein and mRNA expression of cathepsin D in ovarian caneer of different pathological grade or clinical stage was studied. Re,stilts ：The positive rates of cathepsin D mRNA （62.1%） and protein （67.0%） in ovarian cancer group were higher than those in the control group（ P 〈0.01 ）. The positive rates of cathepsin D mRNA （92.9%） and protein （ 100% ） in poorly differentiated ovarian cancer cases were higher than those in the well - differentiated or moderately differentiated cases（ P 〈 0.01 ）. The positive rates of cathepsin D retiNA （91.7%） and protein （ 100% ） of Ⅲ～Ⅳstage ovarian cancer cases were higher than those of Ⅰ～Ⅱ stage cases（ P 〈0.01 ）. The positive rates of cathepsin D mRNA （91.7%） and protein （ 100% ） in metastasis ovarian csncer cases were higher than those in cases vdthout metastasis （ P 〈 0.01 ）. The MVD in the poorly differentiated, Ⅲ～Ⅳstage and metastatic ovarian cancer cases were （45.6±3.6）, （47.8 ± 4.1） and （46.6 ± 3.9 ）, respectively, which were higher than those in the well or moderately differentiated, Ⅰ～Ⅱ stage and no metastasis eases. In ovarian eancer cases with positive cathepsin D mRNA or positive protein detection, the MVD were （35.3 ± 4.1 ） and （34. 2 ± 4.3 ）, which were higher than those in the negative cases. Conclusion：The expression rates of cathepsin D in transcription and protein level in ovarian cancer are higher than those in normal ovar）, which have relationship with the pathological grade, clinieal stage and angiogenesis of ovarian cancer.
出处
《西南国防医药》
CAS
2009年第1期54-57,共4页
Medical Journal of National Defending Forces in Southwest China
