摘要
目的研究子宫肌瘤组织中孕激素受体（ＰＲ）基因的表达情况，从ＰＲ基因的转录及翻译水平探讨米非司酮治疗子宫肌瘤的作用机理。方法采用Ｎｏｒｔｈｅｒｎ印迹法和羟基磷灰石（ＨＡＰ）单点饱和分析法，分别测定２７例单纯手术治疗（单纯手术组）及６例术前口服米非司酮每天２５ｍｇ共３个月（米非司酮组）的子宫肌瘤患者的肌瘤瘤心、瘤边缘及子宫体正常肌组织中ＰＲｍＲＮＡ和ＰＲ蛋白。结果单纯手术组瘤心和瘤边缘中ＰＲｍＲＮＡ及ＰＲ蛋白水平在卵泡期和黄体期均显著高于子宫体肌组织（Ｐ＜０．０１），呈持续高表达，而瘤心和瘤边缘间差异无显著性（Ｐ＞０．０５）；米非司酮组４例术前未停药者，肌瘤及子宫体肌组织中ＰＲｍＲＮＡ和ＰＲ蛋白水平均明显降低，２例术前停药１月者，上述组织中ＰＲｍＲＮＡ与单纯手术者相似，但ＰＲ蛋白仍处于低水平。结论子宫肌瘤中ＰＲ基因呈持续高表达。米非司酮能抑制ＰＲ基因的表达，在ｍＲＮＡ水平上的影响是可逆的，在蛋白水平上的影响持续时间较长。
Objective To determine the expression of progesterone receptor (PR) mRNA and PR protein levels in the myometrium and leiomyomata from untreated and mifepristone pretreated women with leiomyomataand to examine the mechanism of mifepristone treatment on uterine leiomyomata.Methods Expression of PR mRNA and PR protein were determined by Northern blot and HAP of single dose saturated analysis in myometrium and leiomyomata (center and marginal area) from 27 untreated and 6 mifepristone pretreated women with leiomyomata.Results PR mRNA abundance and PR protein levels in both myomatous center and marginal area were significantly greater than those in corporal myometrium ( P <0.01) in both follicular and luteal phases, but similar between myomatous center and marginal area ( P >0.05). 6 cases pretreated with mifepristone 25 mg/day for 3 months were operated, all but one patient displayed a decrease in leiomyomata volume. PR mRNA abundance in both myometruim and leiomyomata (center and marginal area) was significantly decreased in 4 patients continuing mifepristone treatment before the operation but not in the other 2 patients stopping RU486 1 month before operation. PR protein levels in these tissues showed significant decrease in all 6 cases.Conclusion There are overexpression of PRmRNA and PR protein in leiomyomata. One of the mechanism of mifepristone action on decreasing leiomyomata volume may be related to suppression on expression of PR gene. It seems that suppression on transcription of PR gene is reversible, but on translation of PR gene may maintain in a relatively longer period.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
1998年第4期227-231,共5页
Chinese Journal of Obstetrics and Gynecology
关键词
米非司酮
子宫肿瘤
平滑肌瘤
受体
孕酮
Mifepristone Uterine neoplasms Leiomyoma Receptors, progesterone Gene expression
