摘要
对枯草芽孢杆菌(Bacillus Subtilis)的固态发酵工艺进行了研究,包括碳源、氮源、含水量、初始pH、接种时间、接种量等影响因素,并通过单因子试验和正交试验优化,确定了枯草芽孢杆菌的固态发酵工艺为:(1)培养基:麸皮19.5 g,稻谷壳0.5 g,豆粕1.0 g,水22 mL;(2)发酵条件:初始pH为7.0、接种时间20 h,接种量为0.9 mL。照此工艺发酵,枯草芽孢杆菌的活菌数高达435亿个/g。
The influence factors of solid state fermentation process of Bacillus subtilis including carbon, nitrogen, water content, initial pH, inoculum age and inoculum size were studied in the article. The results obtained by using mono-factor experiments and orthodox experiments showed that the optimal solid state fermenta- tion medium was composed of bran 19.5 g, rice crust 0.5 g, soybean 1 g, water 22 mL, and the optimized fermentation conditions were initial pH 7.0, inoculum age of 20 h, 0.9 mL inoculum size. Under the above solid state fermentation process, the viable bacteria counts of Bacillus subtilis could reach 43.5 billion/gram.
出处
《渔业现代化》
2008年第6期48-50,共3页
Fishery Modernization
关键词
枯草芽孢杆菌
固态发酵
活菌数
Bacillus subtilis
solid state fermentation
viable bacteria counts
作者简介
刘唤明（1978～），男，讲师，硕士生，研究方向为发酵工程。E-mail：lhmgdhyds@sina．com
