摘要
以外消旋苯基环氧乙烷为唯一碳源,从土壤中筛选出编号为G7的菌株,能选择性水解外消旋苯基环氧乙烷而得到(S)-苯基环氧乙烷,对映体过量值(e.e)达到99.3%。经鉴定菌种G7属于枯草芽胞杆菌。对该菌株的发酵条件进行了优化,结果表明,当培养基成分为蔗糖10g/L,胰蛋白胨10g/L,Na2HPO4.12H2O2g/L,KH2PO42g/L,MgSO40.2g/L,CaCl20.01g/L,pH6.5,30℃培养25h,环氧化物水解酶活力达到最高值372.2U/mg。
A bacterial strain G7 producing highly enantioselective epoxide hydrolase was isolated from soil. The enantiomeric excess （e. e） value of the styrene oxide formed was 99.3 %. G7 was identified as Bacillus subtilis by comparing its 16S rDNA sequence with data in GenBank. Fermentation conditions for strain G7, including carbon source, nitrogen source, other ions, pH and culture time were studied and the optimum conditions were found to be as follows： sucrose 10g/L, tryptone 10g/L, Na2HPO4·12H2O 2g/L, KHzPO4 2g/L, MggO4 0.2 g/L, CaCl2 0.01 g/L, pH6.5, 30℃, culture time of 25 h. The maximum.observed enzyme activity was 372.2 U/mg under the optimal conditions.
出处
《北京化工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2008年第6期75-79,共5页
Journal of Beijing University of Chemical Technology(Natural Science Edition)
基金
国家'863'计划(2006AA02Z250)
作者简介
男，1983年生．硕士生
通讯联系人 E-mail：zhenggj@mail．buct．edu．cn
