摘要
在M9基础培养基的基础上,以乳糖为诱导剂,对基因重组蛛丝蛋白工程菌pNSR32/BL21(DE3)的发酵培养基进行了优化。利用单因子实验和正交试验优化出表达高分子量重组蛛丝蛋白的最优培养基配方,结果表明:优化的碳源为0.3%的甘油,氮源为3%的酵母膏、0.75%的蛋白胨和0.05%(NH4)2SO4及少量的无机盐。优化培养基有利于菌体的生长和目的蛋白的表达,表达重组蛛丝蛋白达总蛋白量的20%。
Based on M9 culture medium, the concentration of ingredients of culture medium was optimized for the fermentation of pNSR32/BL21 （ DE3 ）, the engineering bacterial with spider silk protein, and lactose as an inducer. The condition of optimum culture medium was obtained for the expression of the high molecular weight recombinant spider silk protein by using orthogonal and individual factor experimental design. The result was showed that the optimum culture medium was consisted of 0.3% glycerol, 3% yeast,0.75% tryptone, 0. 05% （NH4 ）2 SO4 and a little inorganic salt It was confirmed that the optimum culture medium will benefit the growth of bacterial and expression of recombinant spider silk protein. The production level of propose protein will attain at 20% of the total proteins in the fermentation.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2008年第12期41-46,共6页
China Biotechnology
基金
国家自然科学基金(30570956)
福建省发改委项目(2006-781)资助项目
关键词
重组蛛丝蛋白
发酵
乳糖培养基
优化
Recombinant spider silk protein Fermentation Lactose medium Optimization
作者简介
通讯作者，电子信箱：mli@fjnu．edu．cn
