摘要
目的:为研究锌转运体3(ZnT3)在神经系统功能中的作用提供形态学依据。方法:制备带有地高辛标记物的ZnT3反义RNA探针,取雄性SD大鼠脑、脊髓和脊神经节行冷冻切片,作ZnT3 mRNA原位杂交组织化学显色,杂交后用AKP标记的抗地高辛抗体显色。结果:脑内ZnT3 mRNA主要分布于海马结构,包括齿状回颗粒细胞和CA1～CA4区的锥体细胞,在梨状皮质、扣带后皮质中也有分布,显示的细胞较小;脑干中也有ZnT3 mRNA分布,面神经核处分布较多,网状结构中有少量分布;脊髓灰质前角内有少数胞体较大的细胞呈ZnT3 mRNA阳性;脊神经节内的多数神经元呈ZnT3 mRNA强阳性。结论:ZnT3 mRNA主要分布于海马、大脑皮质、杏仁核、脑干网状结构等可塑性较强结构和周围神经神经元中,提示ZnT3可能在学习、记忆、初级感觉传入和肌运动调控等方面发挥重要作用。
Objective： To provide morphological base for studing the effect of Zn transporter 3 （ZnT3） on the nervous system function. Methods： Antisense riboprobes were labeled with digoxigenin dUTP by in vitro transcription of mouse cDNA en- coding ZnT3. The brain, spinal cord and spinal ganglion in adult male rats were removed and sectioned in a cryostat. In situ hybridization was performed on free-floating tissue sections, which were incubated with an alkaline phosphatase-labeled anti digoxigenin antibody, and then incubated with nitroblue tetrazolium salt and 5-bromo 4 chloro-3 indolyl--phosphate toluidinium salt for staining. Results： （1） In the brain, ZnT3 mRNA was mainly distributed in the hippocampus, also in the pyri form cortex and posterior cortex of cingulum. （2） Compared with the reticular formation of the brain stem, ZnT3 mRNA was expressed more in the facial nucleus. （3） Some big cells in the anterior horn of the spinal cord gray mater were ZnT3 mRNA positive. （4） ZnT3 mRNA was strongly expressed in most neurons of the spinal ganglion. Conclusion： ZnT3 mRNA mainly distributes in some strong plastic areas such as the hippocampus, cerebral cortex, amygdaloid nucleus, reticular formation of the brain stem and neurons of the peripheral nerve. It suggests that ZnT3 may play important roles in nervous system function, such as learning, memory, primary sensation, and regulation of muscle movement.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2008年第6期747-750,共4页
Chinese Journal of Anatomy
基金
国家自然科学基金面上项目(39970641)
关键词
锌转运体3
脑
脊髓
脊神经节
原位杂交
Zn transporter 3
brain
spinal cord
spinal ganglion
In situ hybridization
作者简介
Email：liufang．@smmu．edu．cn
通讯作者，Email：xujiajun1963@yahoo．com．cn
