摘要
克隆并分析猪FcRn基因;十二指肠组织提取总RNA,利用设计的引物进行RT-PCR,PCR产物与pMD-19T载体连接后转化JM109感受态细胞,筛选阳性克隆并测序;FcRn阳性克隆两条序列在NCBI上比较,显示序列同猪FcRn(AY740682.1)同源性都为99%。克隆了猪FcRn基因的序列和其剪接变体序列,其剪接变体序列已在GenBank上注册(Accession.EU852582)
To clone and analysis the FcRn gene of pig. Total RNA was extracted from mucosal tissue of duodenum and mRNA sequence of gene were amplified by RT-PCR using two designed primers. The PCR products were ligated into the pMD-19T vector, and then transformed into competent cells of JM109. The sequence was analyzed to identify the recombinant plasmid. Identity analysis showed that the two FcRn nucleotide sequence shared 99 % .The porcine alternatively spliced variant of FcRn gene was successfully cloned in the present study and the sequence of spliced variant has been submitted to GenBank （Accession. EU852582）.
出处
《中国农学通报》
CSCD
2008年第11期27-31,共5页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金资助项目"FcRn在仔猪肠道的表达规律及其与母乳IgG转运的关系"(30671531)
关键词
猪
FCRN
剪接变体
克隆
序列分析
Pig, FcRn, Alternatively spliced variant Molecular Cloning, Sequence Analysis.
作者简介
李海霞，女，1983年出生，硕士研究生，研究方向：泌乳生物学。通信地址：450002郑州河南农业大学牧医工程学院动物生理生化实验室，Email：lhx5162003@yahoo．com．cn。
通讯作者：王艳玲，女，1962年出生，博士，教授，博导，研究方向：泌乳生物学。通信地址：450002河南农业大学牧医工程学院，Tel：0371-63558888，E-mail：wangyanlingl@yahoo．com。
