摘要
目的探讨侧脑室注射乙酰胆碱对大鼠海马学习和空间记忆能力的影响。方法15只雄性Wistar大鼠被随机分为实验组、假手术组和对照组,每组5只。实验组和假手术组动物麻醉后经立体定位侧脑室注射分别给予乙酰胆碱和生理盐水,对照组则只对动物进行麻醉。术后4周观察在Morris水迷宫试验中动物平台逃避潜伏期时间和穿越平台的次数。结果在定位航行试验中,实验组、假手术组和对照组大鼠的逃避潜伏期分别为(6.06±0.85)s、(13.15±2.73)s和(11.77±2.00)s,实验组明显低于假手术组和对照组(P<0.05)。在空间搜索试验中,实验组、假手术组和对照组在60s内穿越平台次数分别为(7.4±0.51)次、(4.2±0.37)次和(3.6±0.51)次,实验组高于假手术组和对照组(P<0.05)。结论侧脑室给予乙酰胆碱可提高成年大鼠海马的学习和空间记忆能力。
Objective To explore the effects of acetylcholine（Ach）on learning and spatial memory in the rat hippocapus. Methods Fifteen male Wistar rats were divided into 3 groups：test group （treated with ACh）, sham group （treated with saline）and control group（no treatment）. ACh and 0.9% -NaCl were injected stereotaxically into the lateral ventricles of rats in test group and sham group, respectively, but nothing was given to the rats in control group except being anaesthetized. After operation for 4 weeks, place navigation task（PNT）and spatial probe task（SPT）were used to detect the time of escaping to the platform within 60 s and the times of crossing over the platform within 60 s, respectively. Results In PNT, the time of escaping to the platform in test group was lower than in sham group and control group[ （6.06 ± 0.85）s vs （13.15 ± 2.73）s and（11. 77 ± 2.00）s, P〈 0.05]. In SPT, the times of crossing over the platform within 60 s were higher in test group than in sham group and control group（7.4±0.51 vs 4.2 ±0. 37 and 3.6 ± 0.51, P 〈 0.05）. Conclusion ACh being injected stereotaxically into the lateral ventricles can improve the ability of rats on learning and memory.
出处
《山西医科大学学报》
CAS
2008年第11期961-964,共4页
Journal of Shanxi Medical University
基金
山西省回国留学人员科研基金资助项目(2007-40)
关键词
乙酰胆碱
学习和记忆
海马
大鼠
acetylcholine
learning and spatial memory
hippocarnpus
rats
作者简介
张文平，男，1970—12生，博士，讲师．
通讯作者，E—mail：cezh2002@yahoo．com
