摘要
目的研究腺病毒介导的人白细胞介素-24(hIL-24)基因选择性诱导喉癌细胞Hep-2凋亡的作用。方法将携带有hIL-24基因的重组复制缺陷型腺病毒(Ad-hIL-24)感染喉癌细胞Hep-2,并以人胚肺成纤维细胞WI-38为对照,采用RT-PCR法检测Hep-2、WI-38细胞中外源性hIL-24基因表达;采用四甲基偶氮唑蓝(MTT)比色法、激光扫描共聚焦显微镜(LSCM)及流式细胞术等方法检测细胞的生长和凋亡情况。结果腺病毒介导的hIL-24基因能在Hep-2细胞和WI-38细胞中表达;MTT法显示Ad-hIL-24能明显抑制喉癌细胞Hep-2的生长,LSCM观察结果表明Ad-hIL-24能促进喉癌细胞Hep-2凋亡,流式细胞术显示Hep-2细胞经Ad-hIL-24感染48h后凋亡率为18.47%,G2/M期细胞百分比为38.9%,显著高于PBS组、Ad-GFP组和WI-38细胞,差异有高度统计学意义(P<0.01)。结论Ad-hIL-24能抑制喉癌细胞Hep-2生长和诱导Hep-2细胞凋亡,但对正常细胞无毒性作用。
Objective To study the effect of Ad-hIL-24 in inducing apoptosis in human laryngocarcinoma cell Hep-2. Methods The human IL-24 gene was transfected into human laryngeal cancer cell line Hep-2 and hUman diploid cell line WI-38 with a replication-incompetent adenovirus vector. The Ad-hIL-24 gene expression was confirmed by RT-PCR in Hep-2 cells and WI-38 cells. The cell growth and the apoptotic effect were tested by MTT assay,Hoechest 33258 staining and laser scanning eonfocal microscopy （LSCM） and flow cytometry. Results RT-PCR confirmed that the Ad-hIL-24 was expressed in Hep-2 cells and WI-38 cells. MTr assay and LSCM test indicated Ad-hlL-24 could induce growth suppression and apoptosis in Hep-2 cells but not in WI-38 cells.Within 48 h after Ad-hIL-24 infection, the apoptosis rate of Hep-2 cells was 18.47%, the Hep-2 cells percentage in G2/M were 38.9%, significantly higher than those in the empty group,uninfected group and WI-38 cell. Conclusion Ad- hIL-24 can selectively inhibit proliferation and induces apoptosis of Hep-2 cells.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2008年第5期706-709,881,共5页
Suzhou University Journal of Medical Science
基金
苏州大学医学发展基金资助项目(NO.EE134517)
关键词
腺病毒
白介素-24
喉癌细胞
凋亡
adenovirus
interleukin-24
laryngocarcinoma cell
apoptosis
作者简介
杨学明（1976-），男，福建仙游人，住院医师，在读医学硕士，研究方向为分子免疫学。
通讯作者：缪竞诚
