摘要
在相同的温度和时间条件下,比较滤纸片法和甘油生理盐水冻存法保存嗜酸乳杆菌的效果。用滤纸片法和甘油生理盐水冻存法,分别进行不同温度的嗜酸乳杆菌保存试验;并于预定时间内,对各处理组分别进行复苏及预定检测。在室温和4℃条件下,用滤纸片法保存的嗜酸乳杆菌出现了生化特性和形态的异常状况。随着保存时间的延长,滤纸片保存法比甘油生理盐水冻存法的嗜酸乳杆菌存活量明显减少。两种方法保存的菌种,均表现为-80℃组的嗜酸乳杆菌存活量明显大于-20℃组。说明在-80℃温度条件下,甘油生理盐水冻存法比滤纸片法具有较大的嗜酸乳杆菌存活量。
The same strain of Lactobacillus acidophilus was conserved by the filter-paper method and the glycerol with normal saline method in different temperature. They were thawed and tested in the time we setted. In the room temperature group and the 4 ℃ group the bacteria in both of the filter-paper method were mutated,and their morphological and biochemical characteristics changed. With the extension of the conservation, the Lactobacillus acidophilus survival amount was larger in the glycerol with normal saline cryopreservation method compared with the filter-paper method. And in both methods, the survival a- mount of the bacterial in the --80℃ group was more than that in the -20℃ group obviously. All the resuits showed that there was the most number of survivorship of the Lactobacillus acidophilus in --80℃ of the glycerol with normal saline cryopreservation method.
出处
《动物医学进展》
CSCD
2008年第11期37-39,共3页
Progress In Veterinary Medicine
关键词
嗜酸乳杆菌
细菌保存
滤纸片法
甘油生理盐水冻存法
Lactobacillus acidophilus
bacterial conservation
filter-paper method
glycerol normal saline cryopreservation method
作者简介
朱志宁（1983-）．女．北京人．硕士研究生．主要从事猪肠道微生态及其调控研究。
通讯作者
