摘要
研究心肌丝裂素活化蛋白激酶（ＭＡＰＫ）活性及其在细胞中的表达，评价ＭＡＰＫ在高血压心肌重构中的作用。方法对３４和５４周龄组的脑卒中型自发性高血压大鼠及正常血压大鼠进行心肌ＭＡＰＫ活性及其免疫组化定位研究。结果高血压大鼠和正常大鼠的心肌重量与体重比显著增加，心肌出现肥大；心肌ＭＡＰＫ活性３４周龄组增加了１．８１倍，５４周龄组增加了１．４６倍。免疫组化研究显示高血压大鼠心肌内有丰富的ＭＡＰＫ表达，且主要分布于心肌间质细胞。结论心肌ＭＡＰＫ激活可能是传递心肌肥大信息的主要通路。
Objective To explore whether myocardial mitogen activated protein kinase (MAPK) play an important role in hypertensive cardiac remodeling. Methods We compared myocardial MAPK activity by phosphocelluar method and its localization and distribution by immunohistology in hypertrophic myocardium in SHRsp at age of 34 weeks (34w group) and 54 weeks (54w group) with age matched WKY served as control. Results SHRsp was compared with WKY. The heart weight to body weight ratio was significantly increased in both groups. Myocardial MAPK activity increased 1.81 fold in the 34w group and 1.46 fold in the 54w group. MAPK expressed abundantly in hypertrophic myocardium, mainly localized in interstitial cells. Conclusion MAPK might be a major pathway of transducing hypertrophic signals.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1997年第9期680-682,共3页
National Medical Journal of China
