摘要
【目的】研究中国荷斯坦奶牛Leptin基因第2外显子的多态性,分析其多态性与产奶性状的关系。【方法】以中国荷斯坦奶牛为材料,利用PCR-SSCP技术对瘦素基因(Leptin)第2外显子的遗传多态性及其与奶牛产奶性状的相关性进行了分析。【结果】中国荷斯坦奶牛Leptin基因第2外显子有2种等位基因A和B,等位基因频率分别为0.697 5和0.302 5,群体多态信息含量为0.333 0,杂合度为0.422 0,A等位基因是群体中的优势等位基因;BB型个体第1,2,3胎的乳脂率和乳蛋白率及第1,2胎的产奶量等产奶性状显著或极显著优于AA型(P<0.01或P<0.05);第1,2,3胎的产奶量及第1,2胎的乳脂率和第2胎的乳蛋白率也显著或极显著优于AB型(P<0.01或P<0.05)。【结论】Leptin基因可用于中国荷期坦奶牛产奶性状的辅助选择。
[Obiective] The polymorphism of exon2 of Leptin gene was studied and its association with milk production traits in Chinese Herstein Cattle was analyzed. [Method] This study was designed to identify the polymorphism of exon 2 of Leptin gene and its relationship with milk production traits in 200 Chinese Holstein by PCR-SSCP. [Result] The frequencies of alleles A and B were 0. 697 5 and 0. 302 5 respectively. Polymorphism information content was 0. 333 0, and heterozygosity was 0. 422 0. The allele A was the dominant allele in Chinese Holstein. Individuals with genotype BB had a significant higher average milk protein percentage and milk fat percentage for all three parities,and a significant higher average milk yield for the first parity and the second parity than that of genotype AA（P〈0.01 or P〈0.05）. They also had a significant higher milk yield for all three parities,milk fat percentage for the first parity and the second par ity,milk protein percentage for the second parity better than that of genotypeAB（P〈0.01 or P〈0.05）. [Conclusion] The results indicated that Leptin gene could be used as the molecular genetic marker to select the milk production traits in cattle.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2008年第11期6-10,共5页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家"十一五"奶业重大专项(2006BAD04A11)
农业科技跨越计划项目(2007-23)
西北农林科技大学拔尖人才支持计划项目
作者简介
刘新武（1981-），男，江苏盐城人，在读硕士，主要从事生物技术在家畜育种中的应用研究。E—mail：lxw1223@126．com
[通讯作者]昝林森（1963-），男，陕西扶风人，教授，博士，博士生导师，主要从事动物生长发育调控及牛的遗传育种与繁殖研究。E-mail：zanls@yahoo．com．cn
