摘要
以美国、阿根廷和转基因大豆标准品为材料,利用设计的特异性引物和探针,建立了实时荧光定量PCR技术检测抗草甘膦转基因豆粕的方法,成功检测出美国和阿根廷抗草甘膦转基因豆粕的内源参照基因lectin、CaMV35S/CTP of EPSPS边界序列和NOS终止子,确定了实时荧光PCR方法检测抗草甘膦转基因豆粕的灵敏度为0.1%。
Using the American and Argentine Soybean Meal and standard samples of genetically modified soybean as baseline materials and utilizing specially designed primers and probes, the research established the qualitative detection method of glyphosate-resistant Genetically Modified Soybean Meal by Real Time Fluorescence PCR method. The method successfully detected the endogenesis gene Lectin, CaMV35S/CTP of EPSPS boundary sequence and NOS terminator in the glyphosate--resistant Genetically Modified Soybean Meal imported from the USA and Rgentina. However, no other gene but the endogenesis gene Lectin is found in Chinese Soybean Meal. The sensitivity of the detection of glyphosate--resistant Genetically Modified Soybean Meal by Real Time Fluorescence PCR method is 0.1%.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2008年第9期143-145,共3页
Food and Fermentation Industries
基金
山东省教育厅资助项目(021155)
关键词
转基因豆粕
实时荧光PCR
检测
Genetically Modified Soybean Meal, Real Time Fluorescence PCR, dtection
作者简介
博士研究生，讲师。
黄亚东副教授为通讯作者。
