摘要
目的研究电磁辐射对家兔小脑谷氨酸受体2(glutamate receptor 2,GluR2)蛋白质表达和磷酸化水平的影响,探讨电磁辐射对小脑运动性学习记忆的神经信号传导通路的损伤特点。方法30只二级日本大耳白家兔随机分为对照组和电磁辐射组(包括辐射后0、3、24和72 h等4个时相组)。辐射组给予平均功率密度为65 mW/cm^2 S波段电磁波持续辐射30 min,测定辐射前和辐射后即刻家兔的肛温并计算比吸收率值;采用Western blot方法检测小脑GIuR2蛋白质表达及其磷酸化水平。结果电磁辐射后0 h家兔肛温升高2.35℃,SAR值为19.00 J/(kg·s);小脑GluR2蛋白质表达水平无显著变化,但GluR2蛋白质磷酸化水平在电磁辐射后0 h显著降低。结论65 mW/cm^2电磁辐射30 min可使家兔机体产生明显热效应,并导致小脑GluR2的磷酸化水平下降,将最终影响运动性学习记忆功能。
Objective To investigate the effects of electromagnetic irradiation on cerebellar glutamate receptor 2 （GluR2） protein expression and phosphorylation in rabbit. Methods Thirty rabbits were divided randomly into control and electromagnetic irradiation group （ including 0, 3, 24 and 72 hour subgroups after irradiation）. Rabbits of electromagnetic irradiation group were exposed to 65 mW/cm^2 S frequency range electromagnetic irradiation for 30 minutes, rectal temperatures were detected immediately after irradiation and specific absorption rate （SAR） values were calculated, the levels of eerebellar GluR2 protein expression and phosphorylation （ ser 880 ） were detected with western blotting. Results After irradiation, rectal temperature of rabbits increased by 2. 35℃ , SAR value was 19. 00 x J/ （ kg · s） , cerebellar GluR2 protein expression had no significant alteration but GluR2 （ser 880 ） phosphorylation reduced significantly. Conclusion After 65 mW/cm^2 of electromagnetic irradiation for 30 minutes, heating effect occurred in rabbits obviously, and cerebellar GluR2 protein phosphorylation （ser 880） decreased significantly. These effects will damage cerebellar motor learning.
出处
《中国预防医学杂志》
CAS
2008年第10期879-882,共4页
Chinese Preventive Medicine
关键词
电磁辐射
小脑
谷氨酸受体2
蛋白质磷酸化
家兔
Electromagnetic irradiation
Cerebellum
Glutamate receptor 2
Phosphorylation. Rabbit
