摘要
为探讨冻害对马铃薯块茎生活力的影响及其快速检测方法,试验设置了5℃、0℃、-5℃、-10℃四个温度梯度,在每个温度下设置1d、2d、4d、8d四个时间梯度,对坝薯9号和坝薯10号两个品种马铃薯块茎进行处理,用红墨水法和氯化三苯基四氮唑(TTC)法检测处理后块茎的生活力,并对处理后的块茎作发芽试验。结果表明,马铃薯块茎生活力用红墨水法和TTC法检测都是可行的,TTC法比红墨水法更灵敏。0℃下放置4d及-5℃下放置1d时,用红墨水法可以检测到马铃薯薯块红色开始明显加深,马铃薯块茎生活力明显下降;而在0℃下放置2d及-5℃下放置1d时,用TTC法检测到马铃薯薯块红色开始明显变浅,马铃薯块茎生活力明显下降。发芽试验结果表明,用红墨水法和TTC法检测估测的发芽率与实际发芽率之间无显著差异,并观察到冻害明显抑制马铃薯根的生长。
Four temperature gradients, 5℃, 0℃, -5℃ and -10℃, and four time gradients, 1 d, 2 d, 4 d and 8 d, under each temperature regime were set to study the effect of cold injury on the tuber of two varieties, Bashu 9 and Bashu 10, and its rapid detection method. In addition, sprout test was made on tubers treated by the two methods. Both methods were feasible for the detection of cold injury of potato tubers, but TTC was more sensitive than the red ink method. When potato chips were treated by the red ink, the red color of potato tuber deepened obviously under 0℃ for 4 d or -5℃ for 1 d, and the potato tuber vitality was declined. When potato chips were treated by TTC, the red color faded away under 0℃ for 2 d or -5℃ for 1 d, and the potato tuber vitality was declined. No significant difference was found between the sprouting percentage estimated by the red ink and TTC methods and the one from sprout test. It was also found that root system was inhibited by cold temperatures.
出处
《中国马铃薯》
2008年第5期261-265,共5页
Chinese Potato Journal
基金
四川省科技厅马铃薯优质高产关键技术研究与产业化示范项目(05NG001-021-2)
关键词
冻害
马铃薯
生活力
快速检测
cold injury
potato
vitality
rapid detection
作者简介
林熊（1984-），男，硕士研究生，主要从事马铃薯栽培与生理研究。
通讯作者：E-mail：wxyrtl@163．com
