摘要
采用PCR-SSCP法对85羽绿壳蛋鸡进行促卵泡激素β亚基(FSHβ)和雌激素受体α亚基(ESRα)基因多态性检测并测序,分析其与早期产蛋性状的关系。基因FSHβ和ESRα都发现了多态,测序结果表明:FSHβpro 2位点有3处SNPs,分别是:A-464G,-450插入碱基A,A-405G。FSHβexon 3有1处碱基突变,即A2447G;ESRα基因5'-UTR区有4处碱基变异,分别是:A-34G,T-76C,T-79C,C-102T。ESRα基因内含子1区发现3处SNPs,分别是:T122C,A155G,C169T。与早期(36周)产蛋关系分析表明,FSHβ基因调控区的AA基因型36周平均产蛋量显著高于AB型(p<0.05),没有发现BB型,外显子3的AA型和AB型开产日龄呈显著相关(p<0.05),也没发现BB型;ESRα基因5'侧翼调控区的CC型和CD型36周平均产蛋量差异显著(p<0.05),而没发现DD型,其他均不显著(p>0.05)。
Follicle-stimulating hormone beta（FSHβ） subunit gene and estrogen receptor alpha（ ESRα ）subunit gene were selected as two candidates to screen the genomic variation by means of PCR-SSCP and sequences mensuration in blue eggshell chicken（85 individuals）, and the associ-ation of FSHβ and ESRα genes polymorphisms with early egg production traits were analyzed. Polymorphisms were detected in FSHβ and ESRα genes, the results showed that three nucleotide variation at A-464G,-450A insertion, A-405G, were identified in FSHβ promotor region, and a nucleotide variation at A2447G in FSHβ exon 3 ; Four SNPs , A-34G, T-76C, T-79C, C-102T, were identified in ESRα 5′ flanking regulation region, and three nucleotide variation at T122C, A155G, C169T in ESRα intron 1. Association analysis of early egg production traits showed that the egg number at 36 weeks of genotype AA was higher than that of AB（p 〈 0.05 ）, genotype BB was not found in FSHβ promotor region;Moreover,in exon 3 site,the age at first egg was also significant difference between AA and AB （p 〈 0.05）, neither genotype BB was found. The egg number at 36 weeks was significant difference between CC and CD genotypes of ESRα 5′flanking regulation region（p 〈 0.05）in blue eggshell chicken, genotype DD was not found. Rest is no difference（p 〉 0.05）.
出处
《华北农学报》
CSCD
北大核心
2008年第5期49-52,共4页
Acta Agriculturae Boreali-Sinica
基金
江苏省农业三项工程项目(SX(2003)046)
泰州市科技发展计划项目(TL0812)
作者简介
周俊（1976～），男，江苏泰兴人，讲师，在读博士，主要从事畜禽生产等方面的研究。
通讯作者：杜文兴（1961-），男，江苏张家港人，副教授，博士，主要从事家禽生产研究。
